Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen.
We have previously shown that immunization with solid matrix-antigen-antibody (SMAA) complexes induces both vigorous humoral and cell-mediated immune responses and have suggested that this method of vaccination may be developed for use in humans, and potentially as a vaccine against AIDS. Here we de...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1992
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| _version_ | 1797094630646349824 |
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| author | Hanke, T Szawlowski, P Randall, R |
| author_facet | Hanke, T Szawlowski, P Randall, R |
| author_sort | Hanke, T |
| collection | OXFORD |
| description | We have previously shown that immunization with solid matrix-antigen-antibody (SMAA) complexes induces both vigorous humoral and cell-mediated immune responses and have suggested that this method of vaccination may be developed for use in humans, and potentially as a vaccine against AIDS. Here we demonstrate that a small oligopeptide can act as a tag for the construction of SMAA complexes using a tag-specific monoclonal antibody and tag-linked antigens. We show that a 14-amino acid oligopeptide, present in the phospho (P) and V proteins of simian virus 5 (SV5), retains its antigenicity when attached to the C terminus of three 'foreign' proteins [p27 and gp110 of simian immunodeficiency virus (SIV) and glutathione S-transferase] such that these proteins can be incorporated into SMAA complexes using a monoclonal antibody (MAb) that was originally raised against the native SV5 P and V proteins. Mice were immunized with SMAA complexes containing recombinant p27-TAG and MAbs have been isolated that recognized native SIV p27. The significance of these results in terms of the development of SMAA complexes as human vaccines is discussed. |
| first_indexed | 2024-03-07T04:16:38Z |
| format | Journal article |
| id | oxford-uuid:c9a14f18-c57f-4db1-b366-346875cc8412 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:16:38Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:c9a14f18-c57f-4db1-b366-346875cc84122022-03-27T07:00:51ZConstruction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c9a14f18-c57f-4db1-b366-346875cc8412EnglishSymplectic Elements at Oxford1992Hanke, TSzawlowski, PRandall, RWe have previously shown that immunization with solid matrix-antigen-antibody (SMAA) complexes induces both vigorous humoral and cell-mediated immune responses and have suggested that this method of vaccination may be developed for use in humans, and potentially as a vaccine against AIDS. Here we demonstrate that a small oligopeptide can act as a tag for the construction of SMAA complexes using a tag-specific monoclonal antibody and tag-linked antigens. We show that a 14-amino acid oligopeptide, present in the phospho (P) and V proteins of simian virus 5 (SV5), retains its antigenicity when attached to the C terminus of three 'foreign' proteins [p27 and gp110 of simian immunodeficiency virus (SIV) and glutathione S-transferase] such that these proteins can be incorporated into SMAA complexes using a monoclonal antibody (MAb) that was originally raised against the native SV5 P and V proteins. Mice were immunized with SMAA complexes containing recombinant p27-TAG and MAbs have been isolated that recognized native SIV p27. The significance of these results in terms of the development of SMAA complexes as human vaccines is discussed. |
| spellingShingle | Hanke, T Szawlowski, P Randall, R Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. |
| title | Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. |
| title_full | Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. |
| title_fullStr | Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. |
| title_full_unstemmed | Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. |
| title_short | Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. |
| title_sort | construction of solid matrix antibody antigen complexes containing simian immunodeficiency virus p27 using tag specific monoclonal antibody and tag linked antigen |
| work_keys_str_mv | AT hanket constructionofsolidmatrixantibodyantigencomplexescontainingsimianimmunodeficiencyvirusp27usingtagspecificmonoclonalantibodyandtaglinkedantigen AT szawlowskip constructionofsolidmatrixantibodyantigencomplexescontainingsimianimmunodeficiencyvirusp27usingtagspecificmonoclonalantibodyandtaglinkedantigen AT randallr constructionofsolidmatrixantibodyantigencomplexescontainingsimianimmunodeficiencyvirusp27usingtagspecificmonoclonalantibodyandtaglinkedantigen |