A study of the influence of hormones on the synthesis of ribonucleic acids and proteins in animal tissues
<p>Part One of this thesis descirbes studies on the counter-current distribution of rapidly-labelled RNA. Other workers had previously reported that several hormones, when injected into rats, altered the counter-current distribution profiles of the rapidly-current distribution profiles of the...
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| Format: | Thesis |
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1968
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| Summary: | <p>Part One of this thesis descirbes studies on the counter-current distribution of rapidly-labelled RNA. Other workers had previously reported that several hormones, when injected into rats, altered the counter-current distribution profiles of the rapidly-current distribution profiles of the rapidly-labelled RNA isolated from their livers. It was hoped to use counter-current distribution, in this thesis, to study the RNA synthesised by mammary glad slices incubated with different hormones.</p> <p>Initial experiments were performed to see if hormones influenced the counter-current distribution profiles of rapidly-labelled RNA formed in lover slices. Double-labelling was employed to eliminate differences in isolation and fractionation of control and experimental RNA: control slices were incubated with (14c) adenine, and experimantal slives with the hormone and (3H) adenine. The slices were then combined for RNA isolation and fractionation and the 14C and 4H in each fraction measured simultaneously.</p> <p>[Please see PDF to view rest of abstract]</p> |
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