Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model.
With the increasing prevalence of artemisinin-resistant malaria parasites, a highly efficacious and durable vaccine for malaria is urgently required. We have developed an experimental virus-vectored vaccine platform based on an envelope-modified baculovirus dual-expression system (emBDES). Here, we...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Nature Publishing Group
2018
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| _version_ | 1797094707956809728 |
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| author | Yoshida, K Iyori, M Blagborough, A Salman, A Dulal, P Sala, K Yamamoto, D Khan, S Janse, C Biswas, S Yoshii, T Yusuf, Y Tokoro, M Hill, A Yoshida, S |
| author_facet | Yoshida, K Iyori, M Blagborough, A Salman, A Dulal, P Sala, K Yamamoto, D Khan, S Janse, C Biswas, S Yoshii, T Yusuf, Y Tokoro, M Hill, A Yoshida, S |
| author_sort | Yoshida, K |
| collection | OXFORD |
| description | With the increasing prevalence of artemisinin-resistant malaria parasites, a highly efficacious and durable vaccine for malaria is urgently required. We have developed an experimental virus-vectored vaccine platform based on an envelope-modified baculovirus dual-expression system (emBDES). Here, we show a conceptually new vaccine platform based on an adenovirus-prime/emBDES-boost heterologous immunization regimen expressing the Plasmodium falciparum circumsporozoite protein (PfCSP). A human adenovirus 5-prime/emBDES-boost heterologous immunization regimen consistently achieved higher sterile protection against transgenic P. berghei sporozoites expressing PfCSP after a mosquito-bite challenge than reverse-ordered or homologous immunization. This high protective efficacy was also achieved with a chimpanzee adenovirus 63-prime/emBDES-boost heterologous immunization regimen against an intravenous sporozoite challenge. Thus, we show that the adenovirus-prime/emBDES-boost heterologous immunization regimen confers sterile protection against sporozoite challenge by two individual routes, providing a promising new malaria vaccine platform for future clinical use. |
| first_indexed | 2024-03-07T04:17:46Z |
| format | Journal article |
| id | oxford-uuid:c9f7f026-f23c-4751-9749-5bba07d64897 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:17:46Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:c9f7f026-f23c-4751-9749-5bba07d648972022-03-27T07:04:05ZAdenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c9f7f026-f23c-4751-9749-5bba07d64897EnglishSymplectic Elements at OxfordNature Publishing Group2018Yoshida, KIyori, MBlagborough, ASalman, ADulal, PSala, KYamamoto, DKhan, SJanse, CBiswas, SYoshii, TYusuf, YTokoro, MHill, AYoshida, SWith the increasing prevalence of artemisinin-resistant malaria parasites, a highly efficacious and durable vaccine for malaria is urgently required. We have developed an experimental virus-vectored vaccine platform based on an envelope-modified baculovirus dual-expression system (emBDES). Here, we show a conceptually new vaccine platform based on an adenovirus-prime/emBDES-boost heterologous immunization regimen expressing the Plasmodium falciparum circumsporozoite protein (PfCSP). A human adenovirus 5-prime/emBDES-boost heterologous immunization regimen consistently achieved higher sterile protection against transgenic P. berghei sporozoites expressing PfCSP after a mosquito-bite challenge than reverse-ordered or homologous immunization. This high protective efficacy was also achieved with a chimpanzee adenovirus 63-prime/emBDES-boost heterologous immunization regimen against an intravenous sporozoite challenge. Thus, we show that the adenovirus-prime/emBDES-boost heterologous immunization regimen confers sterile protection against sporozoite challenge by two individual routes, providing a promising new malaria vaccine platform for future clinical use. |
| spellingShingle | Yoshida, K Iyori, M Blagborough, A Salman, A Dulal, P Sala, K Yamamoto, D Khan, S Janse, C Biswas, S Yoshii, T Yusuf, Y Tokoro, M Hill, A Yoshida, S Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model. |
| title | Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model. |
| title_full | Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model. |
| title_fullStr | Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model. |
| title_full_unstemmed | Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model. |
| title_short | Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model. |
| title_sort | adenovirus prime and baculovirus boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model |
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