Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from t...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Springer Nature
2020
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| _version_ | 1797094752296894464 |
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| author | Kang, W Ha, KS Uhm, H Park, K Lee, JY Hohng, S Kang, C |
| author_facet | Kang, W Ha, KS Uhm, H Park, K Lee, JY Hohng, S Kang, C |
| author_sort | Kang, W |
| collection | OXFORD |
| description | Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time. |
| first_indexed | 2024-03-07T04:18:19Z |
| format | Journal article |
| id | oxford-uuid:ca26dd60-59ef-4625-bd49-2c6f9e9a7812 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:18:19Z |
| publishDate | 2020 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:ca26dd60-59ef-4625-bd49-2c6f9e9a78122022-03-27T07:05:21ZTranscription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNAJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ca26dd60-59ef-4625-bd49-2c6f9e9a7812EnglishSymplectic ElementsSpringer Nature2020Kang, WHa, KSUhm, HPark, KLee, JYHohng, SKang, CDespite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time. |
| spellingShingle | Kang, W Ha, KS Uhm, H Park, K Lee, JY Hohng, S Kang, C Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA |
| title | Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA |
| title_full | Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA |
| title_fullStr | Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA |
| title_full_unstemmed | Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA |
| title_short | Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA |
| title_sort | transcription reinitiation by recycling rna polymerase that diffuses on dna after releasing terminated rna |
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