Automated solid-phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high-performance liquid chromatography.
A bioanalytical method for the determination of atovaquone in 100 microl blood-spots by solid-phase extraction and high-performance liquid chromatography has been developed and validated. Atovaquone was extracted from the sampling paper in 0.2 M phosphoric acid and a structurally similar internal st...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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| _version_ | 1797094817589624832 |
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| author | Lindegårth, N Funding, L Bergqvist, Y |
| author_facet | Lindegårth, N Funding, L Bergqvist, Y |
| author_sort | Lindegårth, N |
| collection | OXFORD |
| description | A bioanalytical method for the determination of atovaquone in 100 microl blood-spots by solid-phase extraction and high-performance liquid chromatography has been developed and validated. Atovaquone was extracted from the sampling paper in 0.2 M phosphoric acid and a structurally similar internal standard was added with acetonitrile before being loaded onto a C8 end-capped solid-phase extraction column. Atovaquone and internal standard were analysed by high-performance liquid chromatography on a C18 J'Sphere ODS-M80 (150 x 4.0 mm) column with mobile phase acetonitrile-phosphate buffer, 0.01 M, pH 7.0 (65:35, v/v) and UV detection at 277 nm. The intra-assay precision was 2.7% at 12.00 microM and 13.5% at 1.00 microM. The inter-assay precision was 3.3% at 12.00 microM and 15.6% at 1.00 microM. The lower limit of quantification was 1.00 microM. The limit of detection was 0.50 microM. |
| first_indexed | 2024-03-07T04:19:14Z |
| format | Journal article |
| id | oxford-uuid:ca73c6b1-fbdf-4f2e-820b-388ac66a4e1a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:19:14Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:ca73c6b1-fbdf-4f2e-820b-388ac66a4e1a2022-03-27T07:07:30ZAutomated solid-phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high-performance liquid chromatography.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ca73c6b1-fbdf-4f2e-820b-388ac66a4e1aEnglishSymplectic Elements at Oxford2001Lindegårth, NFunding, LBergqvist, YA bioanalytical method for the determination of atovaquone in 100 microl blood-spots by solid-phase extraction and high-performance liquid chromatography has been developed and validated. Atovaquone was extracted from the sampling paper in 0.2 M phosphoric acid and a structurally similar internal standard was added with acetonitrile before being loaded onto a C8 end-capped solid-phase extraction column. Atovaquone and internal standard were analysed by high-performance liquid chromatography on a C18 J'Sphere ODS-M80 (150 x 4.0 mm) column with mobile phase acetonitrile-phosphate buffer, 0.01 M, pH 7.0 (65:35, v/v) and UV detection at 277 nm. The intra-assay precision was 2.7% at 12.00 microM and 13.5% at 1.00 microM. The inter-assay precision was 3.3% at 12.00 microM and 15.6% at 1.00 microM. The lower limit of quantification was 1.00 microM. The limit of detection was 0.50 microM. |
| spellingShingle | Lindegårth, N Funding, L Bergqvist, Y Automated solid-phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high-performance liquid chromatography. |
| title | Automated solid-phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high-performance liquid chromatography. |
| title_full | Automated solid-phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high-performance liquid chromatography. |
| title_fullStr | Automated solid-phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high-performance liquid chromatography. |
| title_full_unstemmed | Automated solid-phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high-performance liquid chromatography. |
| title_short | Automated solid-phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high-performance liquid chromatography. |
| title_sort | automated solid phase extraction method for the determination of atovaquone in capillary blood applied onto sampling paper by rapid high performance liquid chromatography |
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