The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization
ASPP1 and ASPP2 are both proteins that interact with p53 and enhance its ability to induce apoptosis by selectively elevating the expression of proapoptotic p53-responsive genes. iASPP(RAI) is a third member of the family that is the most conserved inhibitor of p53-mediated apoptosis. Here, we have...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Springer Nature
2004
|
| _version_ | 1797107074996371456 |
|---|---|
| author | Slee, EA Slee, EA Gillotin, S Bergamaschi, D Royer, C Llanos, S Ali, S Jin, B Trigiante, G Lu, X |
| author_facet | Slee, EA Slee, EA Gillotin, S Bergamaschi, D Royer, C Llanos, S Ali, S Jin, B Trigiante, G Lu, X |
| author_sort | Slee, EA |
| collection | OXFORD |
| description | ASPP1 and ASPP2 are both proteins that interact with p53 and enhance its ability to induce apoptosis by selectively elevating the expression of proapoptotic p53-responsive genes. iASPP(RAI) is a third member of the family that is the most conserved inhibitor of p53-mediated apoptosis. Here, we have described iASPP, a longer form of iASPP(RAI), which at 828 amino acids is more than twice the size of iASPP(RAI). Using two antibodies that recognize both iASPP and iASPP(RAI), we report that this longer form of iASPP is the predominant form of the molecule expressed in cells. Like iASPP(RAI), iASPP also binds to p53 and inhibits apoptosis induced by p53 overexpression. However, whereas iASPP(RAI) is predominantly nuclear, the N-terminus of iASPP is entirely cytoplasmic, and the longer iASPP is located in both the cytoplasm and the nucleus. The effect upon subcellular localization of the longer N-terminus of iASPP means that this new, longer form of the molecule may be subject to greater regulation and provides another layer in the control of p53-induced apoptosis. |
| first_indexed | 2024-03-07T07:09:46Z |
| format | Journal article |
| id | oxford-uuid:ca81475c-edb4-49ab-b575-0e5ea5f4b785 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:09:46Z |
| publishDate | 2004 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:ca81475c-edb4-49ab-b575-0e5ea5f4b7852022-06-09T14:06:09ZThe N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localizationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ca81475c-edb4-49ab-b575-0e5ea5f4b785EnglishSymplectic Elements at OxfordSpringer Nature2004Slee, EASlee, EAGillotin, SBergamaschi, DRoyer, CLlanos, SAli, SJin, BTrigiante, GLu, XASPP1 and ASPP2 are both proteins that interact with p53 and enhance its ability to induce apoptosis by selectively elevating the expression of proapoptotic p53-responsive genes. iASPP(RAI) is a third member of the family that is the most conserved inhibitor of p53-mediated apoptosis. Here, we have described iASPP, a longer form of iASPP(RAI), which at 828 amino acids is more than twice the size of iASPP(RAI). Using two antibodies that recognize both iASPP and iASPP(RAI), we report that this longer form of iASPP is the predominant form of the molecule expressed in cells. Like iASPP(RAI), iASPP also binds to p53 and inhibits apoptosis induced by p53 overexpression. However, whereas iASPP(RAI) is predominantly nuclear, the N-terminus of iASPP is entirely cytoplasmic, and the longer iASPP is located in both the cytoplasm and the nucleus. The effect upon subcellular localization of the longer N-terminus of iASPP means that this new, longer form of the molecule may be subject to greater regulation and provides another layer in the control of p53-induced apoptosis. |
| spellingShingle | Slee, EA Slee, EA Gillotin, S Bergamaschi, D Royer, C Llanos, S Ali, S Jin, B Trigiante, G Lu, X The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization |
| title | The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization |
| title_full | The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization |
| title_fullStr | The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization |
| title_full_unstemmed | The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization |
| title_short | The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization |
| title_sort | n terminus of a novel isoform of human iaspp is required for its cytoplasmic localization |
| work_keys_str_mv | AT sleeea thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT sleeea thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT gillotins thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT bergamaschid thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT royerc thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT llanoss thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT alis thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT jinb thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT trigianteg thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT lux thenterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT sleeea nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT sleeea nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT gillotins nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT bergamaschid nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT royerc nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT llanoss nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT alis nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT jinb nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT trigianteg nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization AT lux nterminusofanovelisoformofhumaniasppisrequiredforitscytoplasmiclocalization |