Recurrent genetic defects on chromosome 5q in myeloid neoplasms
BACKGROUND: Deletion of chromosome 5q (del(5q)) is the most common karyotypic abnormality in myeloid neoplasms. MATERIALS AND METHODS: To define the pathogenic molecular features associated with del(5q), next-generation sequencing was applied to 133 patients with myeloid neoplasms (MDS; N = 69, MDS/...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Impact Journals
2016
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| _version_ | 1797094878434295808 |
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| author | Hosono, N Makishima, H Mahfouz, R Przychodzen, B Yoshida, K Jerez, A LaFramboise, T Polprasert, C Clemente, M Shiraishi, Y Chiba, K Tanaka, H Miyano, S Sanada, M Cui, E Verma, A McDevitt, M List, A Saunthararajah, Y Sekeres, M Boultwood, J Ogawa, S Maciejewski, J |
| author_facet | Hosono, N Makishima, H Mahfouz, R Przychodzen, B Yoshida, K Jerez, A LaFramboise, T Polprasert, C Clemente, M Shiraishi, Y Chiba, K Tanaka, H Miyano, S Sanada, M Cui, E Verma, A McDevitt, M List, A Saunthararajah, Y Sekeres, M Boultwood, J Ogawa, S Maciejewski, J |
| author_sort | Hosono, N |
| collection | OXFORD |
| description | BACKGROUND: Deletion of chromosome 5q (del(5q)) is the most common karyotypic abnormality in myeloid neoplasms. MATERIALS AND METHODS: To define the pathogenic molecular features associated with del(5q), next-generation sequencing was applied to 133 patients with myeloid neoplasms (MDS; N = 69, MDS/MPN; N = 5, sAML; N = 29, pAML; N = 30) with del(5q) as a sole abnormally or a part of complex karyotype and results were compared to molecular features of patients diploid for chr5. FINDINGS: A number of 5q genes with haploinsufficient expression and/or recurrent somatic mutations were identified; for these genes, CSNK1A1 and G3BP1 within the commonly deleted 5q region and DDX41 within a commonly retained region were most commonly affected by somatic mutations. These genes showed consistent haploinsufficiency in deleted cases; low expression/mutations of G3BP1 or DDX41 were associated with poor survival, likely due to decreased cellular function. The most common mutations on other chromosomes in patients with del(5q) included TP53, and mutations of FLT3 (ITD or TKD), NPM1 or TET2 and were mutually exclusive. Serial sequencing allowed for definition of clonal architecture and dynamics, in patients with exome sequencing allelic imbalance for informative SNPs facilitated simultaneous approximation of clonal size of del(5q) and clonal burden for somatic mutations. INTERPRETATION: Our results illuminate the spectrum of molecular defects characteristic of del(5q), their clinical impact and succession of stepwise evolution. |
| first_indexed | 2024-03-07T04:20:01Z |
| format | Journal article |
| id | oxford-uuid:cab5f11e-6c39-44c2-b93f-3d7dd1f4a188 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:20:01Z |
| publishDate | 2016 |
| publisher | Impact Journals |
| record_format | dspace |
| spelling | oxford-uuid:cab5f11e-6c39-44c2-b93f-3d7dd1f4a1882022-03-27T07:09:25ZRecurrent genetic defects on chromosome 5q in myeloid neoplasmsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cab5f11e-6c39-44c2-b93f-3d7dd1f4a188EnglishSymplectic Elements at OxfordImpact Journals2016Hosono, NMakishima, HMahfouz, RPrzychodzen, BYoshida, KJerez, ALaFramboise, TPolprasert, CClemente, MShiraishi, YChiba, KTanaka, HMiyano, SSanada, MCui, EVerma, AMcDevitt, MList, ASaunthararajah, YSekeres, MBoultwood, JOgawa, SMaciejewski, JBACKGROUND: Deletion of chromosome 5q (del(5q)) is the most common karyotypic abnormality in myeloid neoplasms. MATERIALS AND METHODS: To define the pathogenic molecular features associated with del(5q), next-generation sequencing was applied to 133 patients with myeloid neoplasms (MDS; N = 69, MDS/MPN; N = 5, sAML; N = 29, pAML; N = 30) with del(5q) as a sole abnormally or a part of complex karyotype and results were compared to molecular features of patients diploid for chr5. FINDINGS: A number of 5q genes with haploinsufficient expression and/or recurrent somatic mutations were identified; for these genes, CSNK1A1 and G3BP1 within the commonly deleted 5q region and DDX41 within a commonly retained region were most commonly affected by somatic mutations. These genes showed consistent haploinsufficiency in deleted cases; low expression/mutations of G3BP1 or DDX41 were associated with poor survival, likely due to decreased cellular function. The most common mutations on other chromosomes in patients with del(5q) included TP53, and mutations of FLT3 (ITD or TKD), NPM1 or TET2 and were mutually exclusive. Serial sequencing allowed for definition of clonal architecture and dynamics, in patients with exome sequencing allelic imbalance for informative SNPs facilitated simultaneous approximation of clonal size of del(5q) and clonal burden for somatic mutations. INTERPRETATION: Our results illuminate the spectrum of molecular defects characteristic of del(5q), their clinical impact and succession of stepwise evolution. |
| spellingShingle | Hosono, N Makishima, H Mahfouz, R Przychodzen, B Yoshida, K Jerez, A LaFramboise, T Polprasert, C Clemente, M Shiraishi, Y Chiba, K Tanaka, H Miyano, S Sanada, M Cui, E Verma, A McDevitt, M List, A Saunthararajah, Y Sekeres, M Boultwood, J Ogawa, S Maciejewski, J Recurrent genetic defects on chromosome 5q in myeloid neoplasms |
| title | Recurrent genetic defects on chromosome 5q in myeloid neoplasms |
| title_full | Recurrent genetic defects on chromosome 5q in myeloid neoplasms |
| title_fullStr | Recurrent genetic defects on chromosome 5q in myeloid neoplasms |
| title_full_unstemmed | Recurrent genetic defects on chromosome 5q in myeloid neoplasms |
| title_short | Recurrent genetic defects on chromosome 5q in myeloid neoplasms |
| title_sort | recurrent genetic defects on chromosome 5q in myeloid neoplasms |
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