Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes.
In human T lymphocytes the antigen receptor (Ti) is associated non-covalently on the cell surface with the invariant T3 antigen which comprises 3 chains: two glycosylated polypeptides of relative molecular mass 26,000 (Mr 26K) and 21K (gamma and delta) and one non-N-glycosylated polypeptide of Mr 19...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1987
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| _version_ | 1797094888671543296 |
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| author | Cantrell, D Davies, A Londei, M Feldman, M Crumpton, M |
| author_facet | Cantrell, D Davies, A Londei, M Feldman, M Crumpton, M |
| author_sort | Cantrell, D |
| collection | OXFORD |
| description | In human T lymphocytes the antigen receptor (Ti) is associated non-covalently on the cell surface with the invariant T3 antigen which comprises 3 chains: two glycosylated polypeptides of relative molecular mass 26,000 (Mr 26K) and 21K (gamma and delta) and one non-N-glycosylated polypeptide of Mr 19K (epsilon). The proposed function of T3 is to transduce the activation signals delivered via the antigen receptor. Recently we have shown that phorbol esters, which stimulate protein kinase C, can induce phosphorylation of the gamma subunit of the T3 antigen. But the critical question is whether T3 phosphorylation occurs as a normal consequence of immune activation of T lymphocytes. In this respect, it has been shown that immune stimulation of murine T cells results in phosphorylation of Ti-associated polypeptides that may be the functional analogues of the human T3 antigen. We have therefore monitored T3 phosphorylation after exposure of human T cells to antigen or phytohaemagglutinin (PHA). The data show that both stimuli initiate phosphorylation of the gamma subunit of the T3 antigen which indicates that T3 phosphorylation is a physiological response to immune activation. |
| first_indexed | 2024-03-07T04:20:07Z |
| format | Journal article |
| id | oxford-uuid:cabc8f27-6709-46dd-819c-fc0734ef3787 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:20:07Z |
| publishDate | 1987 |
| record_format | dspace |
| spelling | oxford-uuid:cabc8f27-6709-46dd-819c-fc0734ef37872022-03-27T07:09:32ZAssociation of phosphorylation of the T3 antigen with immune activation of T lymphocytes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cabc8f27-6709-46dd-819c-fc0734ef3787EnglishSymplectic Elements at Oxford1987Cantrell, DDavies, ALondei, MFeldman, MCrumpton, MIn human T lymphocytes the antigen receptor (Ti) is associated non-covalently on the cell surface with the invariant T3 antigen which comprises 3 chains: two glycosylated polypeptides of relative molecular mass 26,000 (Mr 26K) and 21K (gamma and delta) and one non-N-glycosylated polypeptide of Mr 19K (epsilon). The proposed function of T3 is to transduce the activation signals delivered via the antigen receptor. Recently we have shown that phorbol esters, which stimulate protein kinase C, can induce phosphorylation of the gamma subunit of the T3 antigen. But the critical question is whether T3 phosphorylation occurs as a normal consequence of immune activation of T lymphocytes. In this respect, it has been shown that immune stimulation of murine T cells results in phosphorylation of Ti-associated polypeptides that may be the functional analogues of the human T3 antigen. We have therefore monitored T3 phosphorylation after exposure of human T cells to antigen or phytohaemagglutinin (PHA). The data show that both stimuli initiate phosphorylation of the gamma subunit of the T3 antigen which indicates that T3 phosphorylation is a physiological response to immune activation. |
| spellingShingle | Cantrell, D Davies, A Londei, M Feldman, M Crumpton, M Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes. |
| title | Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes. |
| title_full | Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes. |
| title_fullStr | Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes. |
| title_full_unstemmed | Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes. |
| title_short | Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes. |
| title_sort | association of phosphorylation of the t3 antigen with immune activation of t lymphocytes |
| work_keys_str_mv | AT cantrelld associationofphosphorylationofthet3antigenwithimmuneactivationoftlymphocytes AT daviesa associationofphosphorylationofthet3antigenwithimmuneactivationoftlymphocytes AT londeim associationofphosphorylationofthet3antigenwithimmuneactivationoftlymphocytes AT feldmanm associationofphosphorylationofthet3antigenwithimmuneactivationoftlymphocytes AT crumptonm associationofphosphorylationofthet3antigenwithimmuneactivationoftlymphocytes |