Relationship between nosocomial Acinetobacter species occurring in two geographical areas (Greece and the UK).
Fifty-two isolates of Acinetobacter spp. obtained from three Greek and one UK hospital, were studied using partial 16 S ribosomal DNA sequence analysis, repetitive extragenic palindromic sequence-based polymerase chain reaction (REP-PCR) mediated fingerprinting and DNA macro-restriction analysis. Th...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2003
|
| _version_ | 1797094978440134656 |
|---|---|
| author | Dimopoulou, I Kartali, S Kartalis, G Manolas, K Simopoulos, K Vargemezis, B Theodoropoulou-Rodiou, G Bowler, I Crook, D |
| author_facet | Dimopoulou, I Kartali, S Kartalis, G Manolas, K Simopoulos, K Vargemezis, B Theodoropoulou-Rodiou, G Bowler, I Crook, D |
| author_sort | Dimopoulou, I |
| collection | OXFORD |
| description | Fifty-two isolates of Acinetobacter spp. obtained from three Greek and one UK hospital, were studied using partial 16 S ribosomal DNA sequence analysis, repetitive extragenic palindromic sequence-based polymerase chain reaction (REP-PCR) mediated fingerprinting and DNA macro-restriction analysis. The aim was twofold: first, to discern the major differences in the population of Acinetobacter spp. between the two countries. Second, to compare a simple PCR-based typing scheme with pulsed-field gel electrophoresis (PFGE). The multi-resistant Greek isolates were within DNA groups 2 and TU13, and clustered into three types both by REP-PCR and PFGE. By contrast, the more susceptible Oxford isolates were heterogeneous on 16 S RNA sequence analysis and distinguishable on typing. The need for studies that elucidate the phylogeny of Acinetobacter spp. inside and outside hospitals are important, as this will help clarify the relationship between organisms that are increasingly recognized as causes of severe infections. |
| first_indexed | 2024-03-07T04:21:26Z |
| format | Journal article |
| id | oxford-uuid:cb26a2d1-266d-4ece-b10a-5f420b2c0e09 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:21:26Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:cb26a2d1-266d-4ece-b10a-5f420b2c0e092022-03-27T07:12:45ZRelationship between nosocomial Acinetobacter species occurring in two geographical areas (Greece and the UK).Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cb26a2d1-266d-4ece-b10a-5f420b2c0e09EnglishSymplectic Elements at Oxford2003Dimopoulou, IKartali, SKartalis, GManolas, KSimopoulos, KVargemezis, BTheodoropoulou-Rodiou, GBowler, ICrook, DFifty-two isolates of Acinetobacter spp. obtained from three Greek and one UK hospital, were studied using partial 16 S ribosomal DNA sequence analysis, repetitive extragenic palindromic sequence-based polymerase chain reaction (REP-PCR) mediated fingerprinting and DNA macro-restriction analysis. The aim was twofold: first, to discern the major differences in the population of Acinetobacter spp. between the two countries. Second, to compare a simple PCR-based typing scheme with pulsed-field gel electrophoresis (PFGE). The multi-resistant Greek isolates were within DNA groups 2 and TU13, and clustered into three types both by REP-PCR and PFGE. By contrast, the more susceptible Oxford isolates were heterogeneous on 16 S RNA sequence analysis and distinguishable on typing. The need for studies that elucidate the phylogeny of Acinetobacter spp. inside and outside hospitals are important, as this will help clarify the relationship between organisms that are increasingly recognized as causes of severe infections. |
| spellingShingle | Dimopoulou, I Kartali, S Kartalis, G Manolas, K Simopoulos, K Vargemezis, B Theodoropoulou-Rodiou, G Bowler, I Crook, D Relationship between nosocomial Acinetobacter species occurring in two geographical areas (Greece and the UK). |
| title | Relationship between nosocomial Acinetobacter species occurring in two geographical areas (Greece and the UK). |
| title_full | Relationship between nosocomial Acinetobacter species occurring in two geographical areas (Greece and the UK). |
| title_fullStr | Relationship between nosocomial Acinetobacter species occurring in two geographical areas (Greece and the UK). |
| title_full_unstemmed | Relationship between nosocomial Acinetobacter species occurring in two geographical areas (Greece and the UK). |
| title_short | Relationship between nosocomial Acinetobacter species occurring in two geographical areas (Greece and the UK). |
| title_sort | relationship between nosocomial acinetobacter species occurring in two geographical areas greece and the uk |
| work_keys_str_mv | AT dimopouloui relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk AT kartalis relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk AT kartalisg relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk AT manolask relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk AT simopoulosk relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk AT vargemezisb relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk AT theodoropoulourodioug relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk AT bowleri relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk AT crookd relationshipbetweennosocomialacinetobacterspeciesoccurringintwogeographicalareasgreeceandtheuk |