Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells.
The energy-transducing cytoplasmic membrane of bacteria contains pumps and antiports maintaining the membrane potential and ion gradients. We have developed a method for rapid, single-cell measurement of the internal sodium concentration ([Na(+)](in)) in Escherichia coli using the sodium ion fluores...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2006
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| _version_ | 1797095041040121856 |
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| author | Lo, C Leake, M Berry, R |
| author_facet | Lo, C Leake, M Berry, R |
| author_sort | Lo, C |
| collection | OXFORD |
| description | The energy-transducing cytoplasmic membrane of bacteria contains pumps and antiports maintaining the membrane potential and ion gradients. We have developed a method for rapid, single-cell measurement of the internal sodium concentration ([Na(+)](in)) in Escherichia coli using the sodium ion fluorescence indicator, Sodium Green. The bacterial flagellar motor is a molecular machine that couples the transmembrane flow of ions, either protons (H(+)) or sodium ions (Na(+)), to flagellar rotation. We used an E. coli strain containing a chimeric flagellar motor with H(+)- and Na(+)-driven components that functions as a sodium motor. Changing external sodium concentration ([Na(+)](ex)) in the range 1-85 mM resulted in changes in [Na(+)](in) between 5-14 mM, indicating a partial homeostasis of internal sodium concentration. There were significant intercell variations in the relationship between [Na(+)](in) and [Na(+)](ex), and the internal sodium concentration in cells not expressing chimeric flagellar motors was 2-3 times lower, indicating that the sodium flux through these motors is a significant fraction of the total sodium flux into the cell. |
| first_indexed | 2024-03-07T04:22:21Z |
| format | Journal article |
| id | oxford-uuid:cb737c5d-a641-48f9-9d8a-2818f00391ea |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:22:21Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:cb737c5d-a641-48f9-9d8a-2818f00391ea2022-03-27T07:14:54ZFluorescence measurement of intracellular sodium concentration in single Escherichia coli cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cb737c5d-a641-48f9-9d8a-2818f00391eaEnglishSymplectic Elements at Oxford2006Lo, CLeake, MBerry, RThe energy-transducing cytoplasmic membrane of bacteria contains pumps and antiports maintaining the membrane potential and ion gradients. We have developed a method for rapid, single-cell measurement of the internal sodium concentration ([Na(+)](in)) in Escherichia coli using the sodium ion fluorescence indicator, Sodium Green. The bacterial flagellar motor is a molecular machine that couples the transmembrane flow of ions, either protons (H(+)) or sodium ions (Na(+)), to flagellar rotation. We used an E. coli strain containing a chimeric flagellar motor with H(+)- and Na(+)-driven components that functions as a sodium motor. Changing external sodium concentration ([Na(+)](ex)) in the range 1-85 mM resulted in changes in [Na(+)](in) between 5-14 mM, indicating a partial homeostasis of internal sodium concentration. There were significant intercell variations in the relationship between [Na(+)](in) and [Na(+)](ex), and the internal sodium concentration in cells not expressing chimeric flagellar motors was 2-3 times lower, indicating that the sodium flux through these motors is a significant fraction of the total sodium flux into the cell. |
| spellingShingle | Lo, C Leake, M Berry, R Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells. |
| title | Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells. |
| title_full | Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells. |
| title_fullStr | Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells. |
| title_full_unstemmed | Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells. |
| title_short | Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells. |
| title_sort | fluorescence measurement of intracellular sodium concentration in single escherichia coli cells |
| work_keys_str_mv | AT loc fluorescencemeasurementofintracellularsodiumconcentrationinsingleescherichiacolicells AT leakem fluorescencemeasurementofintracellularsodiumconcentrationinsingleescherichiacolicells AT berryr fluorescencemeasurementofintracellularsodiumconcentrationinsingleescherichiacolicells |