HIV replicates in cultured human brain cells.
Adherent human embryo brain cells have been infected with HIV. Cells replicating HIV were maintained in culture for seven sequential passes over 7 months and continued to produce HIV during that time. Human embryo brain cells displayed glial-cell morphology and expressed glial fibrillary acidic prot...
| Հիմնական հեղինակներ: | , , , , |
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| Ձևաչափ: | Journal article |
| Լեզու: | English |
| Հրապարակվել է: |
1987
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| _version_ | 1826296832282591232 |
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| author | Christofinis, G Papadaki, L Sattentau, Q Ferns, R Tedder, R |
| author_facet | Christofinis, G Papadaki, L Sattentau, Q Ferns, R Tedder, R |
| author_sort | Christofinis, G |
| collection | OXFORD |
| description | Adherent human embryo brain cells have been infected with HIV. Cells replicating HIV were maintained in culture for seven sequential passes over 7 months and continued to produce HIV during that time. Human embryo brain cells displayed glial-cell morphology and expressed glial fibrillary acidic protein. Electron microscopy showed clusters of virus particles around these cells as well as budding virus. Extracted, infected glial cells revealed bands for three major gag proteins, p18, p24 and p55, in Western blotting. It was not possible to detect CD4 antigen on the surface of these cells by indirect immunofluorescence or alkaline phosphatase staining with CD4 monoclonal antibodies. The results of these experiments indicate that HIV replicates in non-malignant brain cells. This observation strengthens the postulated aetiological link between HIV and the encephalopathy, dementia and other neurological symptoms observed in HIV-infected patients. |
| first_indexed | 2024-03-07T04:22:25Z |
| format | Journal article |
| id | oxford-uuid:cb766a45-f500-4776-9d51-d785c16e9a00 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:22:25Z |
| publishDate | 1987 |
| record_format | dspace |
| spelling | oxford-uuid:cb766a45-f500-4776-9d51-d785c16e9a002022-03-27T07:15:01ZHIV replicates in cultured human brain cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cb766a45-f500-4776-9d51-d785c16e9a00EnglishSymplectic Elements at Oxford1987Christofinis, GPapadaki, LSattentau, QFerns, RTedder, RAdherent human embryo brain cells have been infected with HIV. Cells replicating HIV were maintained in culture for seven sequential passes over 7 months and continued to produce HIV during that time. Human embryo brain cells displayed glial-cell morphology and expressed glial fibrillary acidic protein. Electron microscopy showed clusters of virus particles around these cells as well as budding virus. Extracted, infected glial cells revealed bands for three major gag proteins, p18, p24 and p55, in Western blotting. It was not possible to detect CD4 antigen on the surface of these cells by indirect immunofluorescence or alkaline phosphatase staining with CD4 monoclonal antibodies. The results of these experiments indicate that HIV replicates in non-malignant brain cells. This observation strengthens the postulated aetiological link between HIV and the encephalopathy, dementia and other neurological symptoms observed in HIV-infected patients. |
| spellingShingle | Christofinis, G Papadaki, L Sattentau, Q Ferns, R Tedder, R HIV replicates in cultured human brain cells. |
| title | HIV replicates in cultured human brain cells. |
| title_full | HIV replicates in cultured human brain cells. |
| title_fullStr | HIV replicates in cultured human brain cells. |
| title_full_unstemmed | HIV replicates in cultured human brain cells. |
| title_short | HIV replicates in cultured human brain cells. |
| title_sort | hiv replicates in cultured human brain cells |
| work_keys_str_mv | AT christofinisg hivreplicatesinculturedhumanbraincells AT papadakil hivreplicatesinculturedhumanbraincells AT sattentauq hivreplicatesinculturedhumanbraincells AT fernsr hivreplicatesinculturedhumanbraincells AT tedderr hivreplicatesinculturedhumanbraincells |