Rapid evolution of the embryonically-expressed homeobox gene LEUTX within primates
<p>LEUTX is a homeodomain transcription factor expressed in the very early embryo with a function around embryonic genome activation. The LEUTX gene is found only in eutherian mammals including humans but, unlike the majority of homeobox genes, the encoded amino acid sequence is very different...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Oxford University Press
2023
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| _version_ | 1797110565812830208 |
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| author | Lewin, T Blagrove, JR Holland, P |
| author_facet | Lewin, T Blagrove, JR Holland, P |
| author_sort | Lewin, T |
| collection | OXFORD |
| description | <p>LEUTX is a homeodomain transcription factor expressed in the very early embryo with a function around embryonic genome activation. The LEUTX gene is found only in eutherian mammals including humans but, unlike the majority of homeobox genes, the encoded amino acid sequence is very different between divergent mammalian species. However, whether dynamic evolution has also occurred between closely related mammalian species remains unclear. In this work, we perform a comparative genomics study of LEUTX within the primates, revealing dramatic evolutionary sequence change between closely related species. Positive selection has acted on sites in the LEUTX protein, including six sites within the homeodomain; this suggests that selection has driven changes in the set of downstream targets. Transfection into cell culture followed by transcriptomic analysis reveals small functional differences between human and marmoset LEUTX, suggesting rapid sequence evolution has fine-tuned the role of this homeodomain protein within the primates.</p> |
| first_indexed | 2024-03-07T07:56:35Z |
| format | Journal article |
| id | oxford-uuid:cb8e9b3e-6442-4208-899c-748cd0424d1c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:56:35Z |
| publishDate | 2023 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:cb8e9b3e-6442-4208-899c-748cd0424d1c2023-08-17T11:05:25ZRapid evolution of the embryonically-expressed homeobox gene LEUTX within primatesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cb8e9b3e-6442-4208-899c-748cd0424d1cEnglishSymplectic ElementsOxford University Press 2023Lewin, TBlagrove, JRHolland, P<p>LEUTX is a homeodomain transcription factor expressed in the very early embryo with a function around embryonic genome activation. The LEUTX gene is found only in eutherian mammals including humans but, unlike the majority of homeobox genes, the encoded amino acid sequence is very different between divergent mammalian species. However, whether dynamic evolution has also occurred between closely related mammalian species remains unclear. In this work, we perform a comparative genomics study of LEUTX within the primates, revealing dramatic evolutionary sequence change between closely related species. Positive selection has acted on sites in the LEUTX protein, including six sites within the homeodomain; this suggests that selection has driven changes in the set of downstream targets. Transfection into cell culture followed by transcriptomic analysis reveals small functional differences between human and marmoset LEUTX, suggesting rapid sequence evolution has fine-tuned the role of this homeodomain protein within the primates.</p> |
| spellingShingle | Lewin, T Blagrove, JR Holland, P Rapid evolution of the embryonically-expressed homeobox gene LEUTX within primates |
| title | Rapid evolution of the embryonically-expressed homeobox gene LEUTX within primates |
| title_full | Rapid evolution of the embryonically-expressed homeobox gene LEUTX within primates |
| title_fullStr | Rapid evolution of the embryonically-expressed homeobox gene LEUTX within primates |
| title_full_unstemmed | Rapid evolution of the embryonically-expressed homeobox gene LEUTX within primates |
| title_short | Rapid evolution of the embryonically-expressed homeobox gene LEUTX within primates |
| title_sort | rapid evolution of the embryonically expressed homeobox gene leutx within primates |
| work_keys_str_mv | AT lewint rapidevolutionoftheembryonicallyexpressedhomeoboxgeneleutxwithinprimates AT blagrovejr rapidevolutionoftheembryonicallyexpressedhomeoboxgeneleutxwithinprimates AT hollandp rapidevolutionoftheembryonicallyexpressedhomeoboxgeneleutxwithinprimates |