Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Deacetoxycephalosporin C synthetase (expandase) from Cephalosporium acremonium (Acremonium chrysogenum) was purified to near homogeneity as judged by SDS/polyacrylamide-gel electrophoresis. The enzyme (Mr about 40,000) exhibited a pH optimum around 7.5. It required 2-oxoglutarate (Km 0.04 mM), Fe2+...

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Bibliographic Details
Main Authors:	Baldwin, J, Adlington, R, Coates, J, Crabbe, M, Crouch, N, Keeping, J, Knight, G, Schofield, C, Ting, H, Vallejo, C
Format:	Journal article
Language:	English
Published:	1987

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