Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Deacetoxycephalosporin C synthetase (expandase) from Cephalosporium acremonium (Acremonium chrysogenum) was purified to near homogeneity as judged by SDS/polyacrylamide-gel electrophoresis. The enzyme (Mr about 40,000) exhibited a pH optimum around 7.5. It required 2-oxoglutarate (Km 0.04 mM), Fe2+...

Disgrifiad llawn

Manylion Llyfryddiaeth
Prif Awduron:	Baldwin, J, Adlington, R, Coates, J, Crabbe, M, Crouch, N, Keeping, J, Knight, G, Schofield, C, Ting, H, Vallejo, C
Fformat:	Journal article
Iaith:	English
Cyhoeddwyd:	1987

Eitemau Tebyg

Substrate specificity of cloned deacetoxycephalosporin C/deacetylcephalosporin C synthetase.
gan: Baldwin, J, et al.
Cyhoeddwyd: (1988)

STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C
gan: Baldwin, J, et al.
Cyhoeddwyd: (1987)

Oxidation of deacetylcephalosporin C by deacetoxycephalosporin C/deacetylcephalosporin C synthase.
gan: Baldwin, J, et al.
Cyhoeddwyd: (1992)

Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.
gan: Lloyd, MD, et al.
Cyhoeddwyd: (2004)

ENZYMATIC RING EXPANSION OF PENICILLINS TO CEPHALOSPORINS - SIDE-CHAIN SPECIFICITY
gan: Baldwin, J, et al.
Cyhoeddwyd: (1987)

Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).
gan: Lee, H, et al.
Cyhoeddwyd: (2001)

Adipoyl-6-aminopenicillanic acid is a substrate for deacetoxycephalosporin C synthase (DAOCS)
gan: Shibata, N, et al.
Cyhoeddwyd: (1996)

Probing the penicillin sidechain selectivity of recombinant deacetoxycephalosporin C synthase.
gan: Dubus, A, et al.
Cyhoeddwyd: (2001)

The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase.
gan: Lipscomb, S, et al.
Cyhoeddwyd: (2002)

Studies on the active site of deacetoxycephalosporin C synthase.
gan: Lloyd, MD, et al.
Cyhoeddwyd: (1999)

The mechanism of the enzymatic ring expansion of penicillin N to deacetoxycephalosporin C
gan: Crouch, N, et al.
Cyhoeddwyd: (1988)

Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase. The role of arginine 258.
gan: Lee, H, et al.
Cyhoeddwyd: (2001)

The mechanism of ACV synthetase
gan: Byford, M, et al.
Cyhoeddwyd: (1997)

The Mechanism of ACV Synthetase.
gan: Byford, M, et al.
Cyhoeddwyd: (1997)

Active site mutations of recombinant deacetoxycephalosporin C synthase.
gan: Lee, H, et al.
Cyhoeddwyd: (2002)

Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis.
gan: Lawrence, C, et al.
Cyhoeddwyd: (1996)

Molecular mechanism of the multifunctional enzyme L-delta(alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV) synthetase
gan: Shiau, C, et al.
Cyhoeddwyd: (1995)

Molecular mechanism of the multifunctional enzyme L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV) synthetase.
gan: Shiau, C, et al.
Cyhoeddwyd: (1995)

The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase.
gan: Lee, H, et al.
Cyhoeddwyd: (2003)

The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.
gan: Lee, H, et al.
Cyhoeddwyd: (2000)

AN EFFECTIVE SUBSTITUTE FOR ALPHA-AMINOADIPIC ACID IN THE ENZYMATIC-SYNTHESIS OF PENICILLINS
gan: Baldwin, J, et al.
Cyhoeddwyd: (1987)

Hypoxia-inducible factor prolyl-hydroxylase: purification and assays of PHD2.
gan: Hewitson, K, et al.
Cyhoeddwyd: (2007)

THE ENZYMATIC RING EXPANSION OF PENICILLINS TO CEPHALOSPORINS - SIDE-CHAIN SPECIFICITY
gan: Baldwin, J, et al.
Cyhoeddwyd: (1987)

Photoaffinity labelling of isopenicillin N synthetase.
gan: Baldwin, J, et al.
Cyhoeddwyd: (1990)

STUDIES ON THE MECHANISM OF DEACETOXY DEACETYLCEPHALOSPORIN-C SYNTHASE USING CYCLOPROPYL SUBSTITUTED CEPHALOSPORIN PROBES
gan: Baldwin, J, et al.
Cyhoeddwyd: (1991)

AN EFFICIENT SUBSTITUTE FOR THE ALPHA-AMINOADIPOYL MOIETY OF DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE IN THE ENZYMATIC-SYNTHESIS OF PENICILLINS
gan: Baldwin, J, et al.
Cyhoeddwyd: (1987)

THE ENZYMATIC CONVERSION OF EXOMETHYLENE CEPHALOSPORIN-C INTO DEACETYL CEPHALOSPORIN-C AND THE ROLE OF MOLECULAR-OXYGEN IN CEPHALOSPORIN-C BIOSYNTHESIS
gan: Baldwin, J, et al.
Cyhoeddwyd: (1988)

Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase.
gan: Baldwin, J, et al.
Cyhoeddwyd: (1991)

THE ROLE OF MOLECULAR-OXYGEN IN CEPHALOSPORIN-C BIOSYNTHESIS
gan: Baldwin, J, et al.
Cyhoeddwyd: (1987)

PROLINE 4-HYDROXYLASE - STEREOCHEMICAL COURSE OF THE REACTION
gan: Baldwin, J, et al.
Cyhoeddwyd: (1993)

Photoaffinity labelling of isopenicillin N synthetase by laser-flash photolysis.
gan: Baldwin, J, et al.
Cyhoeddwyd: (1989)

CEPHALOSPORIN-C BIOSYNTHESIS - A BRANCHED PATHWAY SENSITIVE TO A KINETIC ISOTOPE EFFECT
gan: Baldwin, J, et al.
Cyhoeddwyd: (1987)

Studies on deacetoxycephalosporin C synthase support a consensus mechanism for 2-oxoglutarate dependent oxygenases.
gan: Tarhonskaya, H, et al.
Cyhoeddwyd: (2014)

Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus.
gan: Baldwin, J, et al.
Cyhoeddwyd: (1990)

CEPHALOSPORIN-C BIOSYNTHESIS - STEREOCHEMISTRY OF THE INCORPORATION OF D,L,D-ALPHA-AMINODIPOYL-CYSTEINYL-(3S)-[2-2H,4-C-13]VALINE INTO BETA-LACTAM COMPOUNDS
gan: Baldwin, J, et al.
Cyhoeddwyd: (1989)

Structure of proline 3-hydroxylase. Evolution of the family of 2-oxoglutarate dependent oxygenases.
gan: Clifton, I, et al.
Cyhoeddwyd: (2001)

Deacetoxycephalosporin C synthase (expandase): Research progress and application potential
gan: Xiaofan Niu, et al.
Cyhoeddwyd: (2021-12-01)

Oxygen sensing by HIF hydroxylases.
gan: Schofield, C, et al.
Cyhoeddwyd: (2004)

Signalling hypoxia by HIF hydroxylases.
gan: Schofield, C, et al.
Cyhoeddwyd: (2005)

Therapeutic manipulation of the HIF hydroxylases.
gan: Nagel, S, et al.
Cyhoeddwyd: (2010)