Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Deacetoxycephalosporin C synthetase (expandase) from Cephalosporium acremonium (Acremonium chrysogenum) was purified to near homogeneity as judged by SDS/polyacrylamide-gel electrophoresis. The enzyme (Mr about 40,000) exhibited a pH optimum around 7.5. It required 2-oxoglutarate (Km 0.04 mM), Fe2+...

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Bibliografiske detaljer
Main Authors:	Baldwin, J, Adlington, R, Coates, J, Crabbe, M, Crouch, N, Keeping, J, Knight, G, Schofield, C, Ting, H, Vallejo, C
Format:	Journal article
Sprog:	English
Udgivet:	1987

Lignende værker

Substrate specificity of cloned deacetoxycephalosporin C/deacetylcephalosporin C synthetase.
af: Baldwin, J, et al.
Udgivet: (1988)

STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C
af: Baldwin, J, et al.
Udgivet: (1987)

Oxidation of deacetylcephalosporin C by deacetoxycephalosporin C/deacetylcephalosporin C synthase.
af: Baldwin, J, et al.
Udgivet: (1992)

Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.
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Udgivet: (2004)

ENZYMATIC RING EXPANSION OF PENICILLINS TO CEPHALOSPORINS - SIDE-CHAIN SPECIFICITY
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Udgivet: (1987)

Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).
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Udgivet: (2001)

Adipoyl-6-aminopenicillanic acid is a substrate for deacetoxycephalosporin C synthase (DAOCS)
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Udgivet: (1996)

Probing the penicillin sidechain selectivity of recombinant deacetoxycephalosporin C synthase.
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Udgivet: (2001)

The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase.
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Udgivet: (2002)

Studies on the active site of deacetoxycephalosporin C synthase.
af: Lloyd, MD, et al.
Udgivet: (1999)

The mechanism of the enzymatic ring expansion of penicillin N to deacetoxycephalosporin C
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Udgivet: (1988)

Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase. The role of arginine 258.
af: Lee, H, et al.
Udgivet: (2001)

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Udgivet: (1997)

The Mechanism of ACV Synthetase.
af: Byford, M, et al.
Udgivet: (1997)

Active site mutations of recombinant deacetoxycephalosporin C synthase.
af: Lee, H, et al.
Udgivet: (2002)

Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis.
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Udgivet: (1996)

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Udgivet: (1995)

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Udgivet: (1995)

The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase.
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Udgivet: (2003)

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Udgivet: (2000)

AN EFFECTIVE SUBSTITUTE FOR ALPHA-AMINOADIPIC ACID IN THE ENZYMATIC-SYNTHESIS OF PENICILLINS
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Udgivet: (1987)

Hypoxia-inducible factor prolyl-hydroxylase: purification and assays of PHD2.
af: Hewitson, K, et al.
Udgivet: (2007)

THE ENZYMATIC RING EXPANSION OF PENICILLINS TO CEPHALOSPORINS - SIDE-CHAIN SPECIFICITY
af: Baldwin, J, et al.
Udgivet: (1987)

Photoaffinity labelling of isopenicillin N synthetase.
af: Baldwin, J, et al.
Udgivet: (1990)

STUDIES ON THE MECHANISM OF DEACETOXY DEACETYLCEPHALOSPORIN-C SYNTHASE USING CYCLOPROPYL SUBSTITUTED CEPHALOSPORIN PROBES
af: Baldwin, J, et al.
Udgivet: (1991)

AN EFFICIENT SUBSTITUTE FOR THE ALPHA-AMINOADIPOYL MOIETY OF DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE IN THE ENZYMATIC-SYNTHESIS OF PENICILLINS
af: Baldwin, J, et al.
Udgivet: (1987)

THE ENZYMATIC CONVERSION OF EXOMETHYLENE CEPHALOSPORIN-C INTO DEACETYL CEPHALOSPORIN-C AND THE ROLE OF MOLECULAR-OXYGEN IN CEPHALOSPORIN-C BIOSYNTHESIS
af: Baldwin, J, et al.
Udgivet: (1988)

Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase.
af: Baldwin, J, et al.
Udgivet: (1991)

THE ROLE OF MOLECULAR-OXYGEN IN CEPHALOSPORIN-C BIOSYNTHESIS
af: Baldwin, J, et al.
Udgivet: (1987)

PROLINE 4-HYDROXYLASE - STEREOCHEMICAL COURSE OF THE REACTION
af: Baldwin, J, et al.
Udgivet: (1993)

Photoaffinity labelling of isopenicillin N synthetase by laser-flash photolysis.
af: Baldwin, J, et al.
Udgivet: (1989)

CEPHALOSPORIN-C BIOSYNTHESIS - A BRANCHED PATHWAY SENSITIVE TO A KINETIC ISOTOPE EFFECT
af: Baldwin, J, et al.
Udgivet: (1987)

Studies on deacetoxycephalosporin C synthase support a consensus mechanism for 2-oxoglutarate dependent oxygenases.
af: Tarhonskaya, H, et al.
Udgivet: (2014)

Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus.
af: Baldwin, J, et al.
Udgivet: (1990)

CEPHALOSPORIN-C BIOSYNTHESIS - STEREOCHEMISTRY OF THE INCORPORATION OF D,L,D-ALPHA-AMINODIPOYL-CYSTEINYL-(3S)-[2-2H,4-C-13]VALINE INTO BETA-LACTAM COMPOUNDS
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Udgivet: (1989)

Structure of proline 3-hydroxylase. Evolution of the family of 2-oxoglutarate dependent oxygenases.
af: Clifton, I, et al.
Udgivet: (2001)

Deacetoxycephalosporin C synthase (expandase): Research progress and application potential
af: Xiaofan Niu, et al.
Udgivet: (2021-12-01)

Oxygen sensing by HIF hydroxylases.
af: Schofield, C, et al.
Udgivet: (2004)

Signalling hypoxia by HIF hydroxylases.
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Udgivet: (2005)

Therapeutic manipulation of the HIF hydroxylases.
af: Nagel, S, et al.
Udgivet: (2010)