Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Deacetoxycephalosporin C synthetase (expandase) from Cephalosporium acremonium (Acremonium chrysogenum) was purified to near homogeneity as judged by SDS/polyacrylamide-gel electrophoresis. The enzyme (Mr about 40,000) exhibited a pH optimum around 7.5. It required 2-oxoglutarate (Km 0.04 mM), Fe2+...

Full beskrivning

Bibliografiska uppgifter
Huvudupphovsmän: Baldwin, J, Adlington, R, Coates, J, Crabbe, M, Crouch, N, Keeping, J, Knight, G, Schofield, C, Ting, H, Vallejo, C
Materialtyp: Journal article
Språk:English
Publicerad: 1987

Liknande verk

Liknande verk