The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors.
The human T cell receptor for antigen (Ti) has recently been identified on IL-2 dependent T cell clones as a 90 kd disulfide-linked heterodimer comprised of one 49-51 kd alpha (alpha) and one 43 kd beta (beta) chain. These subunits are noncovalently associated with a monomorphic 20-25 kd T3 molecule...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1983
|
| _version_ | 1826296888064737280 |
|---|---|
| author | Acuto, O Hussey, R Fitzgerald, K Protentis, J Meuer, S Schlossman, S Reinherz, E |
| author_facet | Acuto, O Hussey, R Fitzgerald, K Protentis, J Meuer, S Schlossman, S Reinherz, E |
| author_sort | Acuto, O |
| collection | OXFORD |
| description | The human T cell receptor for antigen (Ti) has recently been identified on IL-2 dependent T cell clones as a 90 kd disulfide-linked heterodimer comprised of one 49-51 kd alpha (alpha) and one 43 kd beta (beta) chain. These subunits are noncovalently associated with a monomorphic 20-25 kd T3 molecule. Here, we produce monoclonal antibodies to a human tumor (REX) derived from an earlier stage of thymic differentiation in order to determine whether clonotypic structures are expressed and to define the ontogeny of Ti. The results of SDS-PAGE and peptide map analyses indicate that an homologous T3-associated heterodimer is synthesized and expressed by REX. This glycoprotein shares several peptides in common with clonotypic structures on an IL-2 dependent T cell clone. In addition, similar Ti related molecules appear during intrathymic ontogeny in parallel with surface T3 expression. The latter findings provide the structural basis for the immunological competence observed exclusively within the T3+ thymocyte compartment. |
| first_indexed | 2024-03-07T04:23:15Z |
| format | Journal article |
| id | oxford-uuid:cbbe4608-ae5e-4d85-a2f5-2c0179a0f4ee |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:23:15Z |
| publishDate | 1983 |
| record_format | dspace |
| spelling | oxford-uuid:cbbe4608-ae5e-4d85-a2f5-2c0179a0f4ee2022-03-27T07:17:00ZThe human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cbbe4608-ae5e-4d85-a2f5-2c0179a0f4eeEnglishSymplectic Elements at Oxford1983Acuto, OHussey, RFitzgerald, KProtentis, JMeuer, SSchlossman, SReinherz, EThe human T cell receptor for antigen (Ti) has recently been identified on IL-2 dependent T cell clones as a 90 kd disulfide-linked heterodimer comprised of one 49-51 kd alpha (alpha) and one 43 kd beta (beta) chain. These subunits are noncovalently associated with a monomorphic 20-25 kd T3 molecule. Here, we produce monoclonal antibodies to a human tumor (REX) derived from an earlier stage of thymic differentiation in order to determine whether clonotypic structures are expressed and to define the ontogeny of Ti. The results of SDS-PAGE and peptide map analyses indicate that an homologous T3-associated heterodimer is synthesized and expressed by REX. This glycoprotein shares several peptides in common with clonotypic structures on an IL-2 dependent T cell clone. In addition, similar Ti related molecules appear during intrathymic ontogeny in parallel with surface T3 expression. The latter findings provide the structural basis for the immunological competence observed exclusively within the T3+ thymocyte compartment. |
| spellingShingle | Acuto, O Hussey, R Fitzgerald, K Protentis, J Meuer, S Schlossman, S Reinherz, E The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors. |
| title | The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors. |
| title_full | The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors. |
| title_fullStr | The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors. |
| title_full_unstemmed | The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors. |
| title_short | The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent clones and T cell tumors. |
| title_sort | human t cell receptor appearance in ontogeny and biochemical relationship of alpha and beta subunits on il 2 dependent clones and t cell tumors |
| work_keys_str_mv | AT acutoo thehumantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT husseyr thehumantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT fitzgeraldk thehumantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT protentisj thehumantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT meuers thehumantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT schlossmans thehumantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT reinherze thehumantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT acutoo humantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT husseyr humantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT fitzgeraldk humantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT protentisj humantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT meuers humantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT schlossmans humantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors AT reinherze humantcellreceptorappearanceinontogenyandbiochemicalrelationshipofalphaandbetasubunitsonil2dependentclonesandtcelltumors |