Protein-protein interaction site mapping using NMR-detected mutational scanning.

Protein-protein interaction site mapping using NMR-detected mutational scanning.

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We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of i...

Полное описание

Библиографические подробности
Главные авторы:	Baminger, B, Ludwiczek, M, Kontaxis, G, Knapp, S, Konrat, R
Формат:	Journal article
Язык:	English
Опубликовано:	2007
Предметы:	genetics Reproducibility of Results Protein Interaction Mapping Models, Molecular TCF Transcription Factors beta Catenin Transcription Factor 7-Like 2 Protein Ligands methods Binding, Competitive metabolism Nuclear Magnetic Resonance, Biomolecular Mutation

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