Axonally derived neuregulin-1 is required for remyelination and regeneration after nerve injury in adulthood.
Neuregulin-1 (NRG1) plays a crucial role in axoglial signaling during the development of the peripheral nervous system, but its importance in adulthood after peripheral nerve injury remains unclear. We used single-neuron labeling with inducible Cre-mediated knock-out animals, which enabled visualiza...
| Asıl Yazarlar: | , , , , , , , , , , |
|---|---|
| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
2011
|
| _version_ | 1826296934223052800 |
|---|---|
| author | Fricker, F Lago, N Balarajah, S Tsantoulas, C Tanna, S Zhu, N Fageiry, S Jenkins, M Garratt, A Birchmeier, C Bennett, D |
| author_facet | Fricker, F Lago, N Balarajah, S Tsantoulas, C Tanna, S Zhu, N Fageiry, S Jenkins, M Garratt, A Birchmeier, C Bennett, D |
| author_sort | Fricker, F |
| collection | OXFORD |
| description | Neuregulin-1 (NRG1) plays a crucial role in axoglial signaling during the development of the peripheral nervous system, but its importance in adulthood after peripheral nerve injury remains unclear. We used single-neuron labeling with inducible Cre-mediated knock-out animals, which enabled visualization of a subset of adult myelinated sensory and motoneurons neurons in which Nrg1 was inducibly mutated by tamoxifen treatment. In uninjured mice, NRG1-deficient axons and the associated myelin sheath were normal, and the neuromuscular junction demonstrated normal apposition of presynaptic and postsynaptic components. After sciatic nerve crush, NRG1 ablation resulted in severe defects in remyelination: axons were either hypomyelinated or had no myelin sheath. NRG1-deficient axons were also found to regenerate at a slower rate. After nerve injury, the neuromuscular junction was reinnervated, but excess terminal sprouting was observed. Juxtacrine Neuregulin-1 signaling is therefore dispensable for maintenance of the myelin sheath in adult animals but has a key role in reparative processes after nerve injury. |
| first_indexed | 2024-03-07T04:23:57Z |
| format | Journal article |
| id | oxford-uuid:cbfb3874-51a4-42d1-88a7-a4a912f25112 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:23:57Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:cbfb3874-51a4-42d1-88a7-a4a912f251122022-03-27T07:18:42ZAxonally derived neuregulin-1 is required for remyelination and regeneration after nerve injury in adulthood.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cbfb3874-51a4-42d1-88a7-a4a912f25112EnglishSymplectic Elements at Oxford2011Fricker, FLago, NBalarajah, STsantoulas, CTanna, SZhu, NFageiry, SJenkins, MGarratt, ABirchmeier, CBennett, DNeuregulin-1 (NRG1) plays a crucial role in axoglial signaling during the development of the peripheral nervous system, but its importance in adulthood after peripheral nerve injury remains unclear. We used single-neuron labeling with inducible Cre-mediated knock-out animals, which enabled visualization of a subset of adult myelinated sensory and motoneurons neurons in which Nrg1 was inducibly mutated by tamoxifen treatment. In uninjured mice, NRG1-deficient axons and the associated myelin sheath were normal, and the neuromuscular junction demonstrated normal apposition of presynaptic and postsynaptic components. After sciatic nerve crush, NRG1 ablation resulted in severe defects in remyelination: axons were either hypomyelinated or had no myelin sheath. NRG1-deficient axons were also found to regenerate at a slower rate. After nerve injury, the neuromuscular junction was reinnervated, but excess terminal sprouting was observed. Juxtacrine Neuregulin-1 signaling is therefore dispensable for maintenance of the myelin sheath in adult animals but has a key role in reparative processes after nerve injury. |
| spellingShingle | Fricker, F Lago, N Balarajah, S Tsantoulas, C Tanna, S Zhu, N Fageiry, S Jenkins, M Garratt, A Birchmeier, C Bennett, D Axonally derived neuregulin-1 is required for remyelination and regeneration after nerve injury in adulthood. |
| title | Axonally derived neuregulin-1 is required for remyelination and regeneration after nerve injury in adulthood. |
| title_full | Axonally derived neuregulin-1 is required for remyelination and regeneration after nerve injury in adulthood. |
| title_fullStr | Axonally derived neuregulin-1 is required for remyelination and regeneration after nerve injury in adulthood. |
| title_full_unstemmed | Axonally derived neuregulin-1 is required for remyelination and regeneration after nerve injury in adulthood. |
| title_short | Axonally derived neuregulin-1 is required for remyelination and regeneration after nerve injury in adulthood. |
| title_sort | axonally derived neuregulin 1 is required for remyelination and regeneration after nerve injury in adulthood |
| work_keys_str_mv | AT frickerf axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT lagon axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT balarajahs axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT tsantoulasc axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT tannas axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT zhun axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT fageirys axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT jenkinsm axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT garratta axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT birchmeierc axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood AT bennettd axonallyderivedneuregulin1isrequiredforremyelinationandregenerationafternerveinjuryinadulthood |