Electrospun fibres with hyaluronic acid-chitosan nanoparticles produced by a portable device
Electrospinning is a versatile technique to produce nano/microscale fibrous scaffolds for tissue engineering and drug delivery applications. This research aims to demonstrate that hyaluronic acid-chitosan (HA-CS) nanoparticles can be electrospun together with polycaprolactone (PCL) and gelatine (Ge)...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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MDPI
2020
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| _version_ | 1826297108652621824 |
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| author | Fuenteslópez, CV Ye, H |
| author_facet | Fuenteslópez, CV Ye, H |
| author_sort | Fuenteslópez, CV |
| collection | OXFORD |
| description | Electrospinning is a versatile technique to produce nano/microscale fibrous scaffolds for tissue engineering and drug delivery applications. This research aims to demonstrate that hyaluronic acid-chitosan (HA-CS) nanoparticles can be electrospun together with polycaprolactone (PCL) and gelatine (Ge) fibres using a portable device to create scaffolds for tissue repair. A range of polymer solutions of PCL-gelatine at different weight/volume concentrations and ratios were electrospun and characterised. Fibre-cell interaction (F11 cells) was evaluated based on cell viability and proliferation and, from here, a few polymer blends were electrospun into random or aligned fibre arrangements. HA-CS nanoparticles were synthesised, characterised, and used to functionalise electrospun fibres (8% w/v at 70 PCL:30 Ge), which were chosen based on cell viability. Different concentrations of HA-CS nanoparticles were tested to determine cytotoxicity. A single dosage (1 × 10-2 mg/mL) was associated with higher cell proliferation compared with the cell-only control. This nanoparticle concentration was embedded into the electrospun fibres as either surface modification or blend. Fibres with blended NPs delivered a higher cell viability than unmodified fibres, while NP-coated fibres resulted in a higher cell proliferation (72 h) than the NP-blended ones. These biocompatible scaffolds allow cell attachment, maintain fibre arrangement, promote directional growth and yield higher cell viability. |
| first_indexed | 2024-03-07T04:26:33Z |
| format | Journal article |
| id | oxford-uuid:ccd37eb5-09cf-4ee9-ae2e-6a5f5f72452e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:26:33Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oxford-uuid:ccd37eb5-09cf-4ee9-ae2e-6a5f5f72452e2022-03-27T07:24:33ZElectrospun fibres with hyaluronic acid-chitosan nanoparticles produced by a portable device Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ccd37eb5-09cf-4ee9-ae2e-6a5f5f72452eEnglishSymplectic ElementsMDPI2020Fuenteslópez, CVYe, HElectrospinning is a versatile technique to produce nano/microscale fibrous scaffolds for tissue engineering and drug delivery applications. This research aims to demonstrate that hyaluronic acid-chitosan (HA-CS) nanoparticles can be electrospun together with polycaprolactone (PCL) and gelatine (Ge) fibres using a portable device to create scaffolds for tissue repair. A range of polymer solutions of PCL-gelatine at different weight/volume concentrations and ratios were electrospun and characterised. Fibre-cell interaction (F11 cells) was evaluated based on cell viability and proliferation and, from here, a few polymer blends were electrospun into random or aligned fibre arrangements. HA-CS nanoparticles were synthesised, characterised, and used to functionalise electrospun fibres (8% w/v at 70 PCL:30 Ge), which were chosen based on cell viability. Different concentrations of HA-CS nanoparticles were tested to determine cytotoxicity. A single dosage (1 × 10-2 mg/mL) was associated with higher cell proliferation compared with the cell-only control. This nanoparticle concentration was embedded into the electrospun fibres as either surface modification or blend. Fibres with blended NPs delivered a higher cell viability than unmodified fibres, while NP-coated fibres resulted in a higher cell proliferation (72 h) than the NP-blended ones. These biocompatible scaffolds allow cell attachment, maintain fibre arrangement, promote directional growth and yield higher cell viability. |
| spellingShingle | Fuenteslópez, CV Ye, H Electrospun fibres with hyaluronic acid-chitosan nanoparticles produced by a portable device |
| title | Electrospun fibres with hyaluronic acid-chitosan nanoparticles produced by a portable device |
| title_full | Electrospun fibres with hyaluronic acid-chitosan nanoparticles produced by a portable device |
| title_fullStr | Electrospun fibres with hyaluronic acid-chitosan nanoparticles produced by a portable device |
| title_full_unstemmed | Electrospun fibres with hyaluronic acid-chitosan nanoparticles produced by a portable device |
| title_short | Electrospun fibres with hyaluronic acid-chitosan nanoparticles produced by a portable device |
| title_sort | electrospun fibres with hyaluronic acid chitosan nanoparticles produced by a portable device |
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