Cooperation between C/EBPalpha TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation.
Chromatin remodeling is an important step in promoter activation during cellular lineage commitment and differentiation. We show that the ability of the C/EBPalpha transcription factor to direct adipocyte differentiation of uncommitted fibroblast precursors and to activate SWI/SNF-dependent myeloid-...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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| _version_ | 1797095354878918656 |
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| author | Pedersen, T Kowenz-Leutz, E Leutz, A Nerlov, C |
| author_facet | Pedersen, T Kowenz-Leutz, E Leutz, A Nerlov, C |
| author_sort | Pedersen, T |
| collection | OXFORD |
| description | Chromatin remodeling is an important step in promoter activation during cellular lineage commitment and differentiation. We show that the ability of the C/EBPalpha transcription factor to direct adipocyte differentiation of uncommitted fibroblast precursors and to activate SWI/SNF-dependent myeloid-specific genes depends on a domain, C/EBPalpha transactivation element III (TE-III), that binds the SWI/SNF chromatin remodeling complex. TE-III collaborates with C/EBPalpha TBP/TFIIB interaction motifs during induction of adipogenesis and adipocyte-specific gene expression. These results indicate that C/EBPalpha acts as a lineage-instructive transcription factor through SWI/SNF-dependent modification of the chromatin structure of lineage-specific genes, followed by direct promoter activation via recruitment of the basal transcription-initiation complex, and provide a mechanism by which C/EBPalpha can mediate differentiation along multiple cellular lineages. |
| first_indexed | 2024-03-07T04:26:42Z |
| format | Journal article |
| id | oxford-uuid:cce216e6-1be0-4299-86cd-07c7db9e02ef |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:26:42Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:cce216e6-1be0-4299-86cd-07c7db9e02ef2022-03-27T07:24:56ZCooperation between C/EBPalpha TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cce216e6-1be0-4299-86cd-07c7db9e02efEnglishSymplectic Elements at Oxford2001Pedersen, TKowenz-Leutz, ELeutz, ANerlov, CChromatin remodeling is an important step in promoter activation during cellular lineage commitment and differentiation. We show that the ability of the C/EBPalpha transcription factor to direct adipocyte differentiation of uncommitted fibroblast precursors and to activate SWI/SNF-dependent myeloid-specific genes depends on a domain, C/EBPalpha transactivation element III (TE-III), that binds the SWI/SNF chromatin remodeling complex. TE-III collaborates with C/EBPalpha TBP/TFIIB interaction motifs during induction of adipogenesis and adipocyte-specific gene expression. These results indicate that C/EBPalpha acts as a lineage-instructive transcription factor through SWI/SNF-dependent modification of the chromatin structure of lineage-specific genes, followed by direct promoter activation via recruitment of the basal transcription-initiation complex, and provide a mechanism by which C/EBPalpha can mediate differentiation along multiple cellular lineages. |
| spellingShingle | Pedersen, T Kowenz-Leutz, E Leutz, A Nerlov, C Cooperation between C/EBPalpha TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation. |
| title | Cooperation between C/EBPalpha TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation. |
| title_full | Cooperation between C/EBPalpha TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation. |
| title_fullStr | Cooperation between C/EBPalpha TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation. |
| title_full_unstemmed | Cooperation between C/EBPalpha TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation. |
| title_short | Cooperation between C/EBPalpha TBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation. |
| title_sort | cooperation between c ebpalpha tbp tfiib and swi snf recruiting domains is required for adipocyte differentiation |
| work_keys_str_mv | AT pedersent cooperationbetweencebpalphatbptfiibandswisnfrecruitingdomainsisrequiredforadipocytedifferentiation AT kowenzleutze cooperationbetweencebpalphatbptfiibandswisnfrecruitingdomainsisrequiredforadipocytedifferentiation AT leutza cooperationbetweencebpalphatbptfiibandswisnfrecruitingdomainsisrequiredforadipocytedifferentiation AT nerlovc cooperationbetweencebpalphatbptfiibandswisnfrecruitingdomainsisrequiredforadipocytedifferentiation |