Therapeutic potential of monovalent monoclonal antibodies.
One therapeutic use for monoclonal antibody technology is the elimination of categories of unwanted cells by virtue of their distinct cell surface antigens. The efficiency of cell destruction by complement lysis or opsonization depends on a number of factors such as antibody specificity and isotype...
| Main Authors: | , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1984
|
| _version_ | 1797095412715225088 |
|---|---|
| author | Cobbold, S Waldmann, H |
| author_facet | Cobbold, S Waldmann, H |
| author_sort | Cobbold, S |
| collection | OXFORD |
| description | One therapeutic use for monoclonal antibody technology is the elimination of categories of unwanted cells by virtue of their distinct cell surface antigens. The efficiency of cell destruction by complement lysis or opsonization depends on a number of factors such as antibody specificity and isotype as well as certain properties of the target antigen. In some instances cells can escape destruction by redistributing and eventually losing the antigen-antibody complexes from their surface. This process, known as antigenic modulation, generally depends on bivalent antibody binding. Starting from the observation that rabbit antisera can be made more effective at killing tumour cells if they are first rendered univalent by limited proteolysis, we have now prepared a number of monovalent rat monoclonal antibodies to human cell-surface antigens. We find that these antibodies are no longer able to bring about modulation of their target antigens and have an enhanced facility for lysis with human complement. These special properties should greatly increase the therapeutic potential of monoclonal antibodies. |
| first_indexed | 2024-03-07T04:27:29Z |
| format | Journal article |
| id | oxford-uuid:cd262b24-cee1-469c-8ccc-6361188ca5d4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:27:29Z |
| publishDate | 1984 |
| record_format | dspace |
| spelling | oxford-uuid:cd262b24-cee1-469c-8ccc-6361188ca5d42022-03-27T07:26:42ZTherapeutic potential of monovalent monoclonal antibodies.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cd262b24-cee1-469c-8ccc-6361188ca5d4EnglishSymplectic Elements at Oxford1984Cobbold, SWaldmann, HOne therapeutic use for monoclonal antibody technology is the elimination of categories of unwanted cells by virtue of their distinct cell surface antigens. The efficiency of cell destruction by complement lysis or opsonization depends on a number of factors such as antibody specificity and isotype as well as certain properties of the target antigen. In some instances cells can escape destruction by redistributing and eventually losing the antigen-antibody complexes from their surface. This process, known as antigenic modulation, generally depends on bivalent antibody binding. Starting from the observation that rabbit antisera can be made more effective at killing tumour cells if they are first rendered univalent by limited proteolysis, we have now prepared a number of monovalent rat monoclonal antibodies to human cell-surface antigens. We find that these antibodies are no longer able to bring about modulation of their target antigens and have an enhanced facility for lysis with human complement. These special properties should greatly increase the therapeutic potential of monoclonal antibodies. |
| spellingShingle | Cobbold, S Waldmann, H Therapeutic potential of monovalent monoclonal antibodies. |
| title | Therapeutic potential of monovalent monoclonal antibodies. |
| title_full | Therapeutic potential of monovalent monoclonal antibodies. |
| title_fullStr | Therapeutic potential of monovalent monoclonal antibodies. |
| title_full_unstemmed | Therapeutic potential of monovalent monoclonal antibodies. |
| title_short | Therapeutic potential of monovalent monoclonal antibodies. |
| title_sort | therapeutic potential of monovalent monoclonal antibodies |
| work_keys_str_mv | AT cobbolds therapeuticpotentialofmonovalentmonoclonalantibodies AT waldmannh therapeuticpotentialofmonovalentmonoclonalantibodies |