Effect of filtrate containing Clostridium difficile toxin on rectal mucosa maintained in organ culture.
Rectal biopsies were maintained in organ culture over a 24-hour culture period, with good preservation of histological architecture. A filtrate containing Clostridium difficile toxin significantly inhibited the rise in epithelial alkaline phosphatase activity normally seen during culture. This effec...
| Glavni autori: | , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
1985
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| _version_ | 1826297412971397120 |
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| author | Allan, A Jewell, D |
| author_facet | Allan, A Jewell, D |
| author_sort | Allan, A |
| collection | OXFORD |
| description | Rectal biopsies were maintained in organ culture over a 24-hour culture period, with good preservation of histological architecture. A filtrate containing Clostridium difficile toxin significantly inhibited the rise in epithelial alkaline phosphatase activity normally seen during culture. This effect was abolished by pre-incubation of the filtrate with Clostridium sordellii antitoxin, or heat inactivation. This effect is most probably due to a toxin of C. difficile. The method provides a new quantitative approach to the study of luminal toxins as possible pathogenic agents in idiopathic inflammatory diseases of the colon. |
| first_indexed | 2024-03-07T04:31:14Z |
| format | Journal article |
| id | oxford-uuid:ce634bb4-11f8-41cd-a199-a6db4e9cf379 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:31:14Z |
| publishDate | 1985 |
| record_format | dspace |
| spelling | oxford-uuid:ce634bb4-11f8-41cd-a199-a6db4e9cf3792022-03-27T07:35:14ZEffect of filtrate containing Clostridium difficile toxin on rectal mucosa maintained in organ culture.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ce634bb4-11f8-41cd-a199-a6db4e9cf379EnglishSymplectic Elements at Oxford1985Allan, AJewell, DRectal biopsies were maintained in organ culture over a 24-hour culture period, with good preservation of histological architecture. A filtrate containing Clostridium difficile toxin significantly inhibited the rise in epithelial alkaline phosphatase activity normally seen during culture. This effect was abolished by pre-incubation of the filtrate with Clostridium sordellii antitoxin, or heat inactivation. This effect is most probably due to a toxin of C. difficile. The method provides a new quantitative approach to the study of luminal toxins as possible pathogenic agents in idiopathic inflammatory diseases of the colon. |
| spellingShingle | Allan, A Jewell, D Effect of filtrate containing Clostridium difficile toxin on rectal mucosa maintained in organ culture. |
| title | Effect of filtrate containing Clostridium difficile toxin on rectal mucosa maintained in organ culture. |
| title_full | Effect of filtrate containing Clostridium difficile toxin on rectal mucosa maintained in organ culture. |
| title_fullStr | Effect of filtrate containing Clostridium difficile toxin on rectal mucosa maintained in organ culture. |
| title_full_unstemmed | Effect of filtrate containing Clostridium difficile toxin on rectal mucosa maintained in organ culture. |
| title_short | Effect of filtrate containing Clostridium difficile toxin on rectal mucosa maintained in organ culture. |
| title_sort | effect of filtrate containing clostridium difficile toxin on rectal mucosa maintained in organ culture |
| work_keys_str_mv | AT allana effectoffiltratecontainingclostridiumdifficiletoxinonrectalmucosamaintainedinorganculture AT jewelld effectoffiltratecontainingclostridiumdifficiletoxinonrectalmucosamaintainedinorganculture |