Antibodies to AChE(586-599) distinguish Alzheimer's patients from controls
Antibodies raised against human AChE(586-599), reacted to the immunising peptide and to its cognate BuChE(573-586) peptide, but not to the homologous amyloid beta peptide in ELISA. The Western blot detection of three proteins in post-mortem CSF was abolished by competition with AChE(586-599),; these...
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| Format: | Conference item |
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2003
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| Summary: | Antibodies raised against human AChE(586-599), reacted to the immunising peptide and to its cognate BuChE(573-586) peptide, but not to the homologous amyloid beta peptide in ELISA. The Western blot detection of three proteins in post-mortem CSF was abolished by competition with AChE(586-599),; these proteins were more abundant in AD patient samples. In addition, a-1-antitrypsin (AAT) was found to cause an enhanced signal by specific recruitment of the antibody-peptide complex. This recruitment was more abundant in the AD background, despite equal abundance of AAT in AD and control post-mortem CSF. Oligomeric AChE(586-599) was detected in a sandwich ELISA using a single monoclonal antibody. Using this assay, a positive signal was obtained from healthy hippocampal membranes but not those from an AD background. |
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