Electrochemistry of single enzymes: fluctuations of catalase activities
Dynamic fluctuations of the catalytic ability of single catalase enzymes toward hydrogen peroxide decomposition are observed via the nanoimpact technique. The electrochemical signals of single enzymes show that the catalytic ability of single enzymes can temporarily be much higher than expected from...
| Asıl Yazarlar: | , , , |
|---|---|
| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
American Chemical Society
2018
|
| _version_ | 1826297504526761984 |
|---|---|
| author | Lin, C Sepunaru, L Kätelhön, E Compton, R |
| author_facet | Lin, C Sepunaru, L Kätelhön, E Compton, R |
| author_sort | Lin, C |
| collection | OXFORD |
| description | Dynamic fluctuations of the catalytic ability of single catalase enzymes toward hydrogen peroxide decomposition are observed via the nanoimpact technique. The electrochemical signals of single enzymes show that the catalytic ability of single enzymes can temporarily be much higher than expected from the classical, time-averaged Michaelis–Menten description. By combination of experimental data with a new theoretical model, we interpret the unusual enhancement of the single catalase signal and find that single catalases show large fluctuations of the catalytic ability. |
| first_indexed | 2024-03-07T04:32:39Z |
| format | Journal article |
| id | oxford-uuid:ced9efd2-ccd2-4270-b14f-91fd36879c5c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:32:39Z |
| publishDate | 2018 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:ced9efd2-ccd2-4270-b14f-91fd36879c5c2022-03-27T07:38:27ZElectrochemistry of single enzymes: fluctuations of catalase activitiesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ced9efd2-ccd2-4270-b14f-91fd36879c5cEnglishSymplectic Elements at OxfordAmerican Chemical Society2018Lin, CSepunaru, LKätelhön, ECompton, RDynamic fluctuations of the catalytic ability of single catalase enzymes toward hydrogen peroxide decomposition are observed via the nanoimpact technique. The electrochemical signals of single enzymes show that the catalytic ability of single enzymes can temporarily be much higher than expected from the classical, time-averaged Michaelis–Menten description. By combination of experimental data with a new theoretical model, we interpret the unusual enhancement of the single catalase signal and find that single catalases show large fluctuations of the catalytic ability. |
| spellingShingle | Lin, C Sepunaru, L Kätelhön, E Compton, R Electrochemistry of single enzymes: fluctuations of catalase activities |
| title | Electrochemistry of single enzymes: fluctuations of catalase activities |
| title_full | Electrochemistry of single enzymes: fluctuations of catalase activities |
| title_fullStr | Electrochemistry of single enzymes: fluctuations of catalase activities |
| title_full_unstemmed | Electrochemistry of single enzymes: fluctuations of catalase activities |
| title_short | Electrochemistry of single enzymes: fluctuations of catalase activities |
| title_sort | electrochemistry of single enzymes fluctuations of catalase activities |
| work_keys_str_mv | AT linc electrochemistryofsingleenzymesfluctuationsofcatalaseactivities AT sepunarul electrochemistryofsingleenzymesfluctuationsofcatalaseactivities AT katelhone electrochemistryofsingleenzymesfluctuationsofcatalaseactivities AT comptonr electrochemistryofsingleenzymesfluctuationsofcatalaseactivities |