Development of nanopackaging for storage and transport of loaded lipid nanoparticles
Easily deploying new vaccines globally to combat disease outbreaks has been highlighted as a major necessity by the World Health Organization. RNA-based vaccines using lipid nanoparticles (LNPs) as a drug delivery system were employed to great effect during the recent COVID-19 pandemic. However, LNP...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Chemical Society
2023
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| Subjects: |
| _version_ | 1826310752166739968 |
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| author | Kaur, A Darvill, D Xiang, S Heng, JYY Petrov, PK Hoye, RLZ Chen, R |
| author_facet | Kaur, A Darvill, D Xiang, S Heng, JYY Petrov, PK Hoye, RLZ Chen, R |
| author_sort | Kaur, A |
| collection | OXFORD |
| description | Easily deploying new vaccines globally to combat disease outbreaks has been highlighted as a major necessity by the World Health Organization. RNA-based vaccines using lipid nanoparticles (LNPs) as a drug delivery system were employed to great effect during the recent COVID-19 pandemic. However, LNPs are still unstable at room temperature and agglomerate over time during storage, rendering them ineffective for intracellular delivery. We demonstrate the suitability of nanohole arrays (nanopackaging) as patterned surfaces to separate and store functionalized LNPs (fLNPs) in individual recesses, which can be expanded to other therapeutics. Encapsulating calcein as a model drug, we show through confocal microscopy the effective loading of fLNPs into our nanopackaging for both wet and dry systems. We prove quantifiably pH-mediated capture and subsequent unloading of over 30% of the fLNPs using QCM-D on alumina surfaces altering the pH from 5.5 to 7, displaying controllable storage at the nanoscale. |
| first_indexed | 2024-03-07T07:56:36Z |
| format | Journal article |
| id | oxford-uuid:cf0458be-1c3f-480e-9ae0-9bf1dd70e019 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:56:36Z |
| publishDate | 2023 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:cf0458be-1c3f-480e-9ae0-9bf1dd70e0192023-08-23T16:06:18ZDevelopment of nanopackaging for storage and transport of loaded lipid nanoparticlesJournal articlehttp://purl.org/coar/resource_type/c_545buuid:cf0458be-1c3f-480e-9ae0-9bf1dd70e019Drug deliveryOxidesVaccinationpHPolymersEnglishSymplectic ElementsAmerican Chemical Society2023Kaur, ADarvill, DXiang, SHeng, JYYPetrov, PKHoye, RLZChen, REasily deploying new vaccines globally to combat disease outbreaks has been highlighted as a major necessity by the World Health Organization. RNA-based vaccines using lipid nanoparticles (LNPs) as a drug delivery system were employed to great effect during the recent COVID-19 pandemic. However, LNPs are still unstable at room temperature and agglomerate over time during storage, rendering them ineffective for intracellular delivery. We demonstrate the suitability of nanohole arrays (nanopackaging) as patterned surfaces to separate and store functionalized LNPs (fLNPs) in individual recesses, which can be expanded to other therapeutics. Encapsulating calcein as a model drug, we show through confocal microscopy the effective loading of fLNPs into our nanopackaging for both wet and dry systems. We prove quantifiably pH-mediated capture and subsequent unloading of over 30% of the fLNPs using QCM-D on alumina surfaces altering the pH from 5.5 to 7, displaying controllable storage at the nanoscale. |
| spellingShingle | Drug delivery Oxides Vaccination pH Polymers Kaur, A Darvill, D Xiang, S Heng, JYY Petrov, PK Hoye, RLZ Chen, R Development of nanopackaging for storage and transport of loaded lipid nanoparticles |
| title | Development of nanopackaging for storage and transport of loaded lipid nanoparticles |
| title_full | Development of nanopackaging for storage and transport of loaded lipid nanoparticles |
| title_fullStr | Development of nanopackaging for storage and transport of loaded lipid nanoparticles |
| title_full_unstemmed | Development of nanopackaging for storage and transport of loaded lipid nanoparticles |
| title_short | Development of nanopackaging for storage and transport of loaded lipid nanoparticles |
| title_sort | development of nanopackaging for storage and transport of loaded lipid nanoparticles |
| topic | Drug delivery Oxides Vaccination pH Polymers |
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