Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly
<p>Centrioles are microtubule-based organelles that organise the microtubule network and seed the formation of cilia and flagella. New centrioles assemble through a step-wise process dependent notably on the centriolar protein SAS-5 in <i>Caenorhabditis elegans</i>. SAS-5 and its...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
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American Society for Cell Biology
2018
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| _version_ | 1826297555123699712 |
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| author | Bianchi, S Rogala, KB Dynes, NJ Hilbert, M Leidel, SA Steinmetz, MO Gönczy, P Vakonakis, I |
| author_facet | Bianchi, S Rogala, KB Dynes, NJ Hilbert, M Leidel, SA Steinmetz, MO Gönczy, P Vakonakis, I |
| author_sort | Bianchi, S |
| collection | OXFORD |
| description | <p>Centrioles are microtubule-based organelles that organise the microtubule network and seed the formation of cilia and flagella. New centrioles assemble through a step-wise process dependent notably on the centriolar protein SAS-5 in <i>Caenorhabditis elegans</i>. SAS-5 and its functional homologues in other species form oligomers that bind the centriolar proteins SAS-6 and SAS-4, thereby forming an evolutionarily conserved structural core at the onset of organelle assembly. Here, we report a novel interaction of SAS-5 with microtubules. Microtubule binding requires SAS-5 oligomerisation and a disordered protein segment that overlaps with the SAS-4 binding site. Combined <i>in vitro</i> and <i>in vivo</i> analysis of select mutants reveals that the SAS-5–microtubule interaction facilitates centriole assembly in C. elegans embryos. Our findings lead us to propose that the interdependence of SAS-5 oligomerisation and microtubule binding reflects an avidity mechanism that strengthens SAS-5 associations with other centriole components and, thus, promotes organelle assembly.</p> |
| first_indexed | 2024-03-07T04:33:26Z |
| format | Journal article |
| id | oxford-uuid:cf1ca599-032e-4b65-8327-36de9507dc7d |
| institution | University of Oxford |
| last_indexed | 2024-03-07T04:33:26Z |
| publishDate | 2018 |
| publisher | American Society for Cell Biology |
| record_format | dspace |
| spelling | oxford-uuid:cf1ca599-032e-4b65-8327-36de9507dc7d2022-03-27T07:40:16ZInteraction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assemblyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:cf1ca599-032e-4b65-8327-36de9507dc7dSymplectic Elements at OxfordAmerican Society for Cell Biology2018Bianchi, SRogala, KBDynes, NJHilbert, MLeidel, SASteinmetz, MOGönczy, PVakonakis, I <p>Centrioles are microtubule-based organelles that organise the microtubule network and seed the formation of cilia and flagella. New centrioles assemble through a step-wise process dependent notably on the centriolar protein SAS-5 in <i>Caenorhabditis elegans</i>. SAS-5 and its functional homologues in other species form oligomers that bind the centriolar proteins SAS-6 and SAS-4, thereby forming an evolutionarily conserved structural core at the onset of organelle assembly. Here, we report a novel interaction of SAS-5 with microtubules. Microtubule binding requires SAS-5 oligomerisation and a disordered protein segment that overlaps with the SAS-4 binding site. Combined <i>in vitro</i> and <i>in vivo</i> analysis of select mutants reveals that the SAS-5–microtubule interaction facilitates centriole assembly in C. elegans embryos. Our findings lead us to propose that the interdependence of SAS-5 oligomerisation and microtubule binding reflects an avidity mechanism that strengthens SAS-5 associations with other centriole components and, thus, promotes organelle assembly.</p> |
| spellingShingle | Bianchi, S Rogala, KB Dynes, NJ Hilbert, M Leidel, SA Steinmetz, MO Gönczy, P Vakonakis, I Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly |
| title | Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly |
| title_full | Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly |
| title_fullStr | Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly |
| title_full_unstemmed | Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly |
| title_short | Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly |
| title_sort | interaction between the c elegans centriolar protein sas 5 and microtubules facilitates organelle assembly |
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