Structural determinants of conformationally selective, prion-binding aptamers.
We have recently described the isolation of 2'-fluoropyrimidine-substituted RNA aptamers that bind selectively to disease-associated beta-sheet-rich forms of the prion protein, PrP, from a number of mammalian species. These aptamers inhibit the accumulation of protease-resistant forms of PrP in...
| Hoofdauteurs: | , , , , , |
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| Formaat: | Journal article |
| Taal: | English |
| Gepubliceerd in: |
2004
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| _version_ | 1826297753866600448 |
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| author | Sayer, N Cubin, M Rhie, A Bullock, M Tahiri-Alaoui, A James, W |
| author_facet | Sayer, N Cubin, M Rhie, A Bullock, M Tahiri-Alaoui, A James, W |
| author_sort | Sayer, N |
| collection | OXFORD |
| description | We have recently described the isolation of 2'-fluoropyrimidine-substituted RNA aptamers that bind selectively to disease-associated beta-sheet-rich forms of the prion protein, PrP, from a number of mammalian species. These aptamers inhibit the accumulation of protease-resistant forms of PrP in a prion-seeded, in vitro conversion assay. Here we identify the minimal portions of two of these aptamers that retain binding specificity. We determine their secondary structures by a combination of modeling and solution probing. Finally, we identify an internal site for biotinylation of a minimized, synthetic aptamer and use the resultant reagent in the detection of abnormal forms of PrP in vitro. |
| first_indexed | 2024-03-07T04:36:29Z |
| format | Journal article |
| id | oxford-uuid:d0183eb0-cd50-4263-923c-67d6d02d933c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:36:29Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:d0183eb0-cd50-4263-923c-67d6d02d933c2022-03-27T07:47:35ZStructural determinants of conformationally selective, prion-binding aptamers.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d0183eb0-cd50-4263-923c-67d6d02d933cEnglishSymplectic Elements at Oxford2004Sayer, NCubin, MRhie, ABullock, MTahiri-Alaoui, AJames, WWe have recently described the isolation of 2'-fluoropyrimidine-substituted RNA aptamers that bind selectively to disease-associated beta-sheet-rich forms of the prion protein, PrP, from a number of mammalian species. These aptamers inhibit the accumulation of protease-resistant forms of PrP in a prion-seeded, in vitro conversion assay. Here we identify the minimal portions of two of these aptamers that retain binding specificity. We determine their secondary structures by a combination of modeling and solution probing. Finally, we identify an internal site for biotinylation of a minimized, synthetic aptamer and use the resultant reagent in the detection of abnormal forms of PrP in vitro. |
| spellingShingle | Sayer, N Cubin, M Rhie, A Bullock, M Tahiri-Alaoui, A James, W Structural determinants of conformationally selective, prion-binding aptamers. |
| title | Structural determinants of conformationally selective, prion-binding aptamers. |
| title_full | Structural determinants of conformationally selective, prion-binding aptamers. |
| title_fullStr | Structural determinants of conformationally selective, prion-binding aptamers. |
| title_full_unstemmed | Structural determinants of conformationally selective, prion-binding aptamers. |
| title_short | Structural determinants of conformationally selective, prion-binding aptamers. |
| title_sort | structural determinants of conformationally selective prion binding aptamers |
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