DNA-templated protein arrays for single-molecule imaging.

Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of indi...

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Detalhes bibliográficos
Principais autores: Selmi, D, Adamson, R, Attrill, H, Goddard, A, Gilbert, R, Watts, A, Turberfield, A
Formato: Journal article
Idioma:English
Publicado em: 2011
Descrição
Resumo:Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.