DNA-templated protein arrays for single-molecule imaging.
Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of indi...
| Autors principals: | , , , , , , |
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| Format: | Journal article |
| Idioma: | English |
| Publicat: |
2011
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| _version_ | 1826297755262255104 |
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| author | Selmi, D Adamson, R Attrill, H Goddard, A Gilbert, R Watts, A Turberfield, A |
| author_facet | Selmi, D Adamson, R Attrill, H Goddard, A Gilbert, R Watts, A Turberfield, A |
| author_sort | Selmi, D |
| collection | OXFORD |
| description | Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules. |
| first_indexed | 2024-03-07T04:36:30Z |
| format | Journal article |
| id | oxford-uuid:d019b834-a8ad-45d5-a842-6d8fc0f04d0d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:36:30Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:d019b834-a8ad-45d5-a842-6d8fc0f04d0d2022-03-27T07:47:39ZDNA-templated protein arrays for single-molecule imaging.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d019b834-a8ad-45d5-a842-6d8fc0f04d0dEnglishSymplectic Elements at Oxford2011Selmi, DAdamson, RAttrill, HGoddard, AGilbert, RWatts, ATurberfield, ASingle-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules. |
| spellingShingle | Selmi, D Adamson, R Attrill, H Goddard, A Gilbert, R Watts, A Turberfield, A DNA-templated protein arrays for single-molecule imaging. |
| title | DNA-templated protein arrays for single-molecule imaging. |
| title_full | DNA-templated protein arrays for single-molecule imaging. |
| title_fullStr | DNA-templated protein arrays for single-molecule imaging. |
| title_full_unstemmed | DNA-templated protein arrays for single-molecule imaging. |
| title_short | DNA-templated protein arrays for single-molecule imaging. |
| title_sort | dna templated protein arrays for single molecule imaging |
| work_keys_str_mv | AT selmid dnatemplatedproteinarraysforsinglemoleculeimaging AT adamsonr dnatemplatedproteinarraysforsinglemoleculeimaging AT attrillh dnatemplatedproteinarraysforsinglemoleculeimaging AT goddarda dnatemplatedproteinarraysforsinglemoleculeimaging AT gilbertr dnatemplatedproteinarraysforsinglemoleculeimaging AT wattsa dnatemplatedproteinarraysforsinglemoleculeimaging AT turberfielda dnatemplatedproteinarraysforsinglemoleculeimaging |