The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications
Acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. Multidrug resistant A. baumannii has risen rapidly in Vietnam, where colistin is becoming the dru...
| Váldodahkkit: | , , , , , , , , |
|---|---|
| Materiálatiipa: | Journal article |
| Giella: | English |
| Almmustuhtton: |
Nature Publishing Group
2016
|
| _version_ | 1826297769237676032 |
|---|---|
| author | Thi Khanh Nhu, N Riordan, D Do Hoang Nhu, T Thanh, D Thwaites, G Huong Lan, N Wren, B Baker, S Stabler, R |
| author_facet | Thi Khanh Nhu, N Riordan, D Do Hoang Nhu, T Thanh, D Thwaites, G Huong Lan, N Wren, B Baker, S Stabler, R |
| author_sort | Thi Khanh Nhu, N |
| collection | OXFORD |
| description | Acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. Multidrug resistant A. baumannii has risen rapidly in Vietnam, where colistin is becoming the drug of last resort for many infections. In this study we generated spontaneous colistin resistant progeny (up to >256 μg/μl) from four colistin susceptible Vietnamese isolates and one susceptible reference strain (MIC <1.5 μg/μl). Whole genome sequencing was used to identify single nucleotide mutations that could be attributed to the reduced colistin susceptibility. We identified six lpxACD and three pmrB mutations, the majority of which were novel. In addition, we identified further mutations in six A. baumannii genes (vacJ, pldA, ttg2C, pheS and conserved hypothetical protein) that we hypothesise have a role in reduced colistin susceptibility. This study has identified additional mutations that may be associated with colistin resistance through novel resistance mechanisms. Our work further demonstrates how rapidly A. baumannii can generate resistance to a last resort antimicrobial and highlights the need for improved surveillance to identified A. baumannii with an extensive drug resistance profile. |
| first_indexed | 2024-03-07T04:36:42Z |
| format | Journal article |
| id | oxford-uuid:d02b61ef-18a8-4cdb-85f0-5440dc21b89d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:36:42Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:d02b61ef-18a8-4cdb-85f0-5440dc21b89d2022-03-27T07:48:06ZThe induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implicationsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d02b61ef-18a8-4cdb-85f0-5440dc21b89dEnglishSymplectic Elements at OxfordNature Publishing Group2016Thi Khanh Nhu, NRiordan, DDo Hoang Nhu, TThanh, DThwaites, GHuong Lan, NWren, BBaker, SStabler, RAcinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. Multidrug resistant A. baumannii has risen rapidly in Vietnam, where colistin is becoming the drug of last resort for many infections. In this study we generated spontaneous colistin resistant progeny (up to >256 μg/μl) from four colistin susceptible Vietnamese isolates and one susceptible reference strain (MIC <1.5 μg/μl). Whole genome sequencing was used to identify single nucleotide mutations that could be attributed to the reduced colistin susceptibility. We identified six lpxACD and three pmrB mutations, the majority of which were novel. In addition, we identified further mutations in six A. baumannii genes (vacJ, pldA, ttg2C, pheS and conserved hypothetical protein) that we hypothesise have a role in reduced colistin susceptibility. This study has identified additional mutations that may be associated with colistin resistance through novel resistance mechanisms. Our work further demonstrates how rapidly A. baumannii can generate resistance to a last resort antimicrobial and highlights the need for improved surveillance to identified A. baumannii with an extensive drug resistance profile. |
| spellingShingle | Thi Khanh Nhu, N Riordan, D Do Hoang Nhu, T Thanh, D Thwaites, G Huong Lan, N Wren, B Baker, S Stabler, R The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title | The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_full | The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_fullStr | The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_full_unstemmed | The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_short | The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications |
| title_sort | induction and identification of novel colistin resistance mutations in acinetobacter baumannii and their implications |
| work_keys_str_mv | AT thikhanhnhun theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT riordand theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT dohoangnhut theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT thanhd theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT thwaitesg theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT huonglann theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT wrenb theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT bakers theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT stablerr theinductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT thikhanhnhun inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT riordand inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT dohoangnhut inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT thanhd inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT thwaitesg inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT huonglann inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT wrenb inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT bakers inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications AT stablerr inductionandidentificationofnovelcolistinresistancemutationsinacinetobacterbaumanniiandtheirimplications |