Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme.
The final step in the biosynthesis of the plant signaling molecule ethylene is catalyzed by 1-aminocyclopropane-1-carboxylic acid oxidase (ACCO). ACCO requires bicarbonate as an activator and catalyzes the oxidation of ACC to give ethylene, CO2, and HCN. We report crystal structures of ACCO in apo-f...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2004
|
| _version_ | 1797096075012603904 |
|---|---|
| author | Zhang, Z Ren, J Clifton, I Schofield, C |
| author_facet | Zhang, Z Ren, J Clifton, I Schofield, C |
| author_sort | Zhang, Z |
| collection | OXFORD |
| description | The final step in the biosynthesis of the plant signaling molecule ethylene is catalyzed by 1-aminocyclopropane-1-carboxylic acid oxidase (ACCO). ACCO requires bicarbonate as an activator and catalyzes the oxidation of ACC to give ethylene, CO2, and HCN. We report crystal structures of ACCO in apo-form (2.1 A resolution) and complexed with Fe(II) (2.55 A) or Co(II) (2.4 A). The active site contains a single Fe(II) ligated by three residues (His177, Asp179, and His234), and it is relatively open compared to those of the 2-oxoglutarate oxygenases. The side chains of Arg175 and Arg244, proposed to be involved in binding bicarbonate, project away from the active site, but conformational changes may allow either or both to enter the active site. The structures will form a basis for future mechanistic and inhibition studies. |
| first_indexed | 2024-03-07T04:36:52Z |
| format | Journal article |
| id | oxford-uuid:d03be2af-9307-47a6-990c-085a7f50267e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:36:52Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:d03be2af-9307-47a6-990c-085a7f50267e2022-03-27T07:48:32ZCrystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d03be2af-9307-47a6-990c-085a7f50267eEnglishSymplectic Elements at Oxford2004Zhang, ZRen, JClifton, ISchofield, CThe final step in the biosynthesis of the plant signaling molecule ethylene is catalyzed by 1-aminocyclopropane-1-carboxylic acid oxidase (ACCO). ACCO requires bicarbonate as an activator and catalyzes the oxidation of ACC to give ethylene, CO2, and HCN. We report crystal structures of ACCO in apo-form (2.1 A resolution) and complexed with Fe(II) (2.55 A) or Co(II) (2.4 A). The active site contains a single Fe(II) ligated by three residues (His177, Asp179, and His234), and it is relatively open compared to those of the 2-oxoglutarate oxygenases. The side chains of Arg175 and Arg244, proposed to be involved in binding bicarbonate, project away from the active site, but conformational changes may allow either or both to enter the active site. The structures will form a basis for future mechanistic and inhibition studies. |
| spellingShingle | Zhang, Z Ren, J Clifton, I Schofield, C Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme. |
| title | Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme. |
| title_full | Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme. |
| title_fullStr | Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme. |
| title_full_unstemmed | Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme. |
| title_short | Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme. |
| title_sort | crystal structure and mechanistic implications of 1 aminocyclopropane 1 carboxylic acid oxidase the ethylene forming enzyme |
| work_keys_str_mv | AT zhangz crystalstructureandmechanisticimplicationsof1aminocyclopropane1carboxylicacidoxidasetheethyleneformingenzyme AT renj crystalstructureandmechanisticimplicationsof1aminocyclopropane1carboxylicacidoxidasetheethyleneformingenzyme AT cliftoni crystalstructureandmechanisticimplicationsof1aminocyclopropane1carboxylicacidoxidasetheethyleneformingenzyme AT schofieldc crystalstructureandmechanisticimplicationsof1aminocyclopropane1carboxylicacidoxidasetheethyleneformingenzyme |