Towards high-throughput flim for protein-protein interaction screening of live cells and tissue microarrays

Towards high-throughput flim for protein-protein interaction screening of live cells and tissue microarrays

Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells...

书目详细资料
Main Authors:	Barber, P, Pierce, G, Ameer-Beg, S, Matthews, DR, Carlin, L, Keppler, M, Kelleher, M, Festy, F, Gillett, C, Springall, R, Ng, T, Vojnovic, B, IEEE
格式:	Conference item
出版:	2008

相似书籍

Dynamic imaging of protein-protein interactions by MP-FLIM
由: Ameer-Beg, S, et al.
出版: (2005)

A high-content screening platform utilizing polarization anisotropy and FLIM microscopy
由: Matthews, DR, et al.
出版: (2008)

Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein-protein interactions using global analysis
由: Barber, P, et al.
出版: (2009)

Multiphoton-FLIM quantification of the EGFP-mRFP1 FRET pair for localization of membrane receptor-kinase interactions.
由: Peter, M, et al.
出版: (2005)

Deep-tissue multi-photon fluorescence lifetime microscopy for intravital imaging of protein-protein interactions
由: Fruhwirth, G, et al.
出版: (2009)

Global and pixel kinetic data analysis for FRET detection by multi-photon time-domain FLIM
由: Barber, P, et al.
出版: (2005)

A multi-functional imaging approach to high-content protein interaction screening
由: Matthews, DR, et al.
出版: (2012)

Effect of phosphorylation on EGFR dimer stability probed by single-molecule dynamics and FRET/FLIM
由: Coban, O, et al.
出版: (2015)

A multi-functional imaging approach to high-content protein interaction screening.
由: Daniel R Matthews, et al.
出版: (2012-01-01)

Imaging protein-protein interactions in cell motility using fluorescence resonance energy transfer (FRET).
由: Parsons, M, et al.
出版: (2004)

The potential of optical proteomic technologies to individualize prognosis and guide rational treatment for cancer patients
由: Kelleher, M, et al.
出版: (2009)

The potential of optical proteomic technologies to individualize prognosis and guide rational treatment for cancer patients.
由: Kelleher, M, et al.
出版: (2009)

Non fitting based FRET-FLIM analysis approaches applied to quantify protein-protein interactions in live cells
由: Padilla-Parra, S, et al.
出版: (2011)

Non fitting based FRET-FLIM analysis approaches applied to quantify protein-protein interactions in live cells
由: Padilla-Parra, S, et al.
出版: (2011)

Integrating receptor signal inputs that influence small Rho GTPase activation dynamics at the immunological synapse.
由: Makrogianneli, K, et al.
出版: (2009)

HER2-HER3 dimer quantification by FLIM-FRET predicts breast cancer metastatic relapse independently of HER2 IHC status
由: Vojnovic, B, et al.
出版: (2016)

Measuring Small-molecule Inhibition of Protein Interactions in Live Cells Using FLIM-FRET
由: James Pemberton, et al.
出版: (2019-10-01)

Practical and reliable FRET/FLIM pair of fluorescent proteins
由: Shemiakina Irina I, et al.
出版: (2009-03-01)

Time-lapse FRET microscopy using fluorescence anisotropy.
由: Matthews, DR, et al.
出版: (2010)

Robust Bayesian fluorescence lifetime estimation, decay model selection and instrument response determination for low-intensity FLIM imaging
由: Rowley, M, et al.
出版: (2016)

Correction: robust Bayesian fluorescence lifetime estimation, decay model selection and instrument response determination for low-intensity FLIM imaging
由: Rowley, M, et al.
出版: (2016)

Signal-dependent turnover of the bacterial flagellar switch protein FliM
由: Delalez, N, et al.
出版: (2010)

Signal-dependent turnover of the bacterial flagellar switch protein FliM.
由: Delalez, N, et al.
出版: (2010)

Characterisation of red fluorescent protein FLIM properties and comparison with novel StayGold live cell imaging
由: Tripal Philipp, et al.
出版: (2024-01-01)

Wide-Field Multi-Parameter FLIM: long-term minimal invasive observation of proteins in living cells.
由: Marco Vitali, et al.
出版: (2011-01-01)

A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Forster resonance energy transfer with GFP.
由: Ganesan, S, et al.
出版: (2006)

Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.
由: Padilla-Parra, S, et al.
出版: (2009)

Multispectral multiplexed confocal FLIM for live cell imaging
由: Morgan Richards, et al.
出版: (2024-01-01)

How Forster resonance energy transfer imaging improves the understanding of protein interaction networks in cancer biology.
由: Fruhwirth, G, et al.
出版: (2011)

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers.
由: Waterhouse, B, et al.
出版: (2011)

Robust Bayesian Fluorescence Lifetime Estimation, Decay Model Selection and Instrument Response Determination for Low-Intensity FLIM Imaging.
由: Mark I Rowley, et al.
出版: (2016-01-01)

Optimizing FRET-FLIM Labeling Conditions to Detect Nuclear Protein Interactions at Native Expression Levels in Living Arabidopsis Roots
由: Yuchen Long, et al.
出版: (2018-05-01)

Correction: Robust Bayesian Fluorescence Lifetime Estimation, Decay Model Selection and Instrument Response Determination for Low-Intensity FLIM Imaging.
由: Mark I Rowley, et al.
出版: (2016-01-01)

Shining light on the stability of metal thiosemicarbazonate complexes in living cells by FLIM
由: Waghorn, P, et al.
出版: (2013)

Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles
由: Quinten Coucke, et al.
出版: (2023-09-01)

Improving the Stability of Protein–Protein Interaction Assay FlimPIA Using a Thermostabilized Firefly Luciferase
由: Yuki Ohmuro-Matsuyama, et al.
出版: (2021-11-01)

NOVEL OPTICAL PROTEOMICS MOLECULARLY PROFILES BREAST CANCER PATIENTS LEADING TO INDIVIDUALISED PROGNOSIS AND TAILORED TREATMENT
由: Kelleher, M, et al.
出版: (2009)

Thin Flims Vacuum
由: International Symposium on Thin Film Technology (1976 : University of Uppasala,Sweden)
出版: (1976)

FLIM microscopy in biology and medicine /
由: Periasamy, Ammasi, et al.
出版: (c201)

Intracellular signaling in the NF Kappa B activation pathway following oxidative stress measured by fluorescence lifetime imaging of fluorescent proteins
由: Roberts, SA, et al.
出版: (2002)