A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle.
The human malaria parasite Plasmodium falciparum is responsible for the majority of malaria-related deaths. Tools allowing the study of the basic biology of P. falciparum throughout the life cycle are critical to the development of new strategies to target the parasite within both human and mosquito...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Public Library of Science
2010
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| _version_ | 1797096247722508288 |
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| author | Talman, A Blagborough, A Sinden, R |
| author_facet | Talman, A Blagborough, A Sinden, R |
| author_sort | Talman, A |
| collection | OXFORD |
| description | The human malaria parasite Plasmodium falciparum is responsible for the majority of malaria-related deaths. Tools allowing the study of the basic biology of P. falciparum throughout the life cycle are critical to the development of new strategies to target the parasite within both human and mosquito hosts. We here present 3D7HT-GFP, a strain of P. falciparum constitutively expressing the Green Fluorescent Protein (GFP) throughout the life cycle, which has retained its capacity to complete sporogonic development. The GFP expressing cassette was inserted in the Pf47 locus. Using this transgenic strain, parasite tracking and population dynamics studies in mosquito stages and exo-erythrocytic schizogony is greatly facilitated. The development of 3D7HT-GFP will permit a deeper understanding of the biology of parasite-host vector interactions, and facilitate the development of high-throughput malaria transmission assays and thus aid development of new intervention strategies against both parasite and mosquito. |
| first_indexed | 2024-03-07T04:39:09Z |
| format | Journal article |
| id | oxford-uuid:d105e39f-3445-4808-b60a-e2d7ccc6bcf1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:39:09Z |
| publishDate | 2010 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:d105e39f-3445-4808-b60a-e2d7ccc6bcf12022-03-27T07:54:01ZA Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d105e39f-3445-4808-b60a-e2d7ccc6bcf1EnglishSymplectic Elements at OxfordPublic Library of Science2010Talman, ABlagborough, ASinden, RThe human malaria parasite Plasmodium falciparum is responsible for the majority of malaria-related deaths. Tools allowing the study of the basic biology of P. falciparum throughout the life cycle are critical to the development of new strategies to target the parasite within both human and mosquito hosts. We here present 3D7HT-GFP, a strain of P. falciparum constitutively expressing the Green Fluorescent Protein (GFP) throughout the life cycle, which has retained its capacity to complete sporogonic development. The GFP expressing cassette was inserted in the Pf47 locus. Using this transgenic strain, parasite tracking and population dynamics studies in mosquito stages and exo-erythrocytic schizogony is greatly facilitated. The development of 3D7HT-GFP will permit a deeper understanding of the biology of parasite-host vector interactions, and facilitate the development of high-throughput malaria transmission assays and thus aid development of new intervention strategies against both parasite and mosquito. |
| spellingShingle | Talman, A Blagborough, A Sinden, R A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle. |
| title | A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle. |
| title_full | A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle. |
| title_fullStr | A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle. |
| title_full_unstemmed | A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle. |
| title_short | A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle. |
| title_sort | plasmodium falciparum strain expressing gfp throughout the parasite s life cycle |
| work_keys_str_mv | AT talmana aplasmodiumfalciparumstrainexpressinggfpthroughouttheparasiteslifecycle AT blagborougha aplasmodiumfalciparumstrainexpressinggfpthroughouttheparasiteslifecycle AT sindenr aplasmodiumfalciparumstrainexpressinggfpthroughouttheparasiteslifecycle AT talmana plasmodiumfalciparumstrainexpressinggfpthroughouttheparasiteslifecycle AT blagborougha plasmodiumfalciparumstrainexpressinggfpthroughouttheparasiteslifecycle AT sindenr plasmodiumfalciparumstrainexpressinggfpthroughouttheparasiteslifecycle |