Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy.
A system devised for tissue typing the human leukocyte antigen-A (HLA-A) locus from genomic DNA by the polymerase chain reaction (PCR) has been used to investigate abnormalities of HLA expression in a panel of 30 cell lines derived from colorectal adenocarcinomas, by comparison of the HLA-A locus ge...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1993
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| _version_ | 1826298159095087104 |
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| author | Browning, M Krausa, P Rowan, A Hill, AB Bicknell, D Bodmer, J Bodmer, W |
| author_facet | Browning, M Krausa, P Rowan, A Hill, AB Bicknell, D Bodmer, J Bodmer, W |
| author_sort | Browning, M |
| collection | OXFORD |
| description | A system devised for tissue typing the human leukocyte antigen-A (HLA-A) locus from genomic DNA by the polymerase chain reaction (PCR) has been used to investigate abnormalities of HLA expression in a panel of 30 cell lines derived from colorectal adenocarcinomas, by comparison of the HLA-A locus genotype with surface expression of HLA. Eleven cell lines gave single HLA-A locus specificity on PCR typing, suggesting that loss of HLA alleles is a common abnormality. In one of these cell lines the loss of an HLA-A locus allele was confirmed by comparison with DNA from a lymphoblastoid B cell line derived from the same patient. In three cell lines, loss of expression of an HLA-A locus determinant was observed in spite of the presence of the relevant allele in genomic DNA. Three cell lines showed absent HLA expression associated with failure to express beta 2-microglobulin. These data indicate that at least three independent mechanisms were involved in the loss of HLA expression on the colorectal tumor cell lines. |
| first_indexed | 2024-03-07T04:42:36Z |
| format | Journal article |
| id | oxford-uuid:d229f506-c980-4b9e-af0e-b7f07ea1e297 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:42:36Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | oxford-uuid:d229f506-c980-4b9e-af0e-b7f07ea1e2972022-03-27T08:01:57ZLoss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d229f506-c980-4b9e-af0e-b7f07ea1e297EnglishSymplectic Elements at Oxford1993Browning, MKrausa, PRowan, AHill, ABBicknell, DBodmer, JBodmer, WA system devised for tissue typing the human leukocyte antigen-A (HLA-A) locus from genomic DNA by the polymerase chain reaction (PCR) has been used to investigate abnormalities of HLA expression in a panel of 30 cell lines derived from colorectal adenocarcinomas, by comparison of the HLA-A locus genotype with surface expression of HLA. Eleven cell lines gave single HLA-A locus specificity on PCR typing, suggesting that loss of HLA alleles is a common abnormality. In one of these cell lines the loss of an HLA-A locus allele was confirmed by comparison with DNA from a lymphoblastoid B cell line derived from the same patient. In three cell lines, loss of expression of an HLA-A locus determinant was observed in spite of the presence of the relevant allele in genomic DNA. Three cell lines showed absent HLA expression associated with failure to express beta 2-microglobulin. These data indicate that at least three independent mechanisms were involved in the loss of HLA expression on the colorectal tumor cell lines. |
| spellingShingle | Browning, M Krausa, P Rowan, A Hill, AB Bicknell, D Bodmer, J Bodmer, W Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy. |
| title | Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy. |
| title_full | Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy. |
| title_fullStr | Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy. |
| title_full_unstemmed | Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy. |
| title_short | Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy. |
| title_sort | loss of human leukocyte antigen expression on colorectal tumor cell lines implications for anti tumor immunity and immunotherapy |
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