Improving depth in phosphoproteomics by using a strong cation exchange-weak anion exchange-reversed phase multidimensional separation approach.

Improving depth in phosphoproteomics by using a strong cation exchange-weak anion exchange-reversed phase multidimensional separation approach.

Several enrichment and separation strategies are available that allow nearly pure phosphopeptide pools to be created. These phosphopeptide pools are too complex to be completely unraveled by RP-LC-MS analysis alone. Here, we implement weak anion exchange (WAX) chromatography as an additional, comple...

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Detalles Bibliográficos
Autores principales: Hennrich, M, Groenewold, V, Kops, G, Heck, A, Mohammed, S
Formato: Journal article
Lenguaje:English
Publicado: 2011

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