Improving depth in phosphoproteomics by using a strong cation exchange-weak anion exchange-reversed phase multidimensional separation approach.

Several enrichment and separation strategies are available that allow nearly pure phosphopeptide pools to be created. These phosphopeptide pools are too complex to be completely unraveled by RP-LC-MS analysis alone. Here, we implement weak anion exchange (WAX) chromatography as an additional, comple...

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Autors principals: Hennrich, M, Groenewold, V, Kops, G, Heck, A, Mohammed, S
Format: Journal article
Idioma:English
Publicat: 2011