Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.
Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to th...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Jezik: | English |
| Izdano: |
2012
|
| _version_ | 1826298170298073088 |
|---|---|
| author | Botcherby, E Smith, C Kohl, M Débarre, D Booth, M Juškaitis, R Paulsen, O Wilson, T |
| author_facet | Botcherby, E Smith, C Kohl, M Débarre, D Booth, M Juškaitis, R Paulsen, O Wilson, T |
| author_sort | Botcherby, E |
| collection | OXFORD |
| description | Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level. |
| first_indexed | 2024-03-07T04:42:46Z |
| format | Journal article |
| id | oxford-uuid:d238af80-3c7b-4a58-a9db-76a9aeb587da |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:42:46Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:d238af80-3c7b-4a58-a9db-76a9aeb587da2022-03-27T08:02:19ZAberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d238af80-3c7b-4a58-a9db-76a9aeb587daEnglishSymplectic Elements at Oxford2012Botcherby, ESmith, CKohl, MDébarre, DBooth, MJuškaitis, RPaulsen, OWilson, TMultiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level. |
| spellingShingle | Botcherby, E Smith, C Kohl, M Débarre, D Booth, M Juškaitis, R Paulsen, O Wilson, T Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. |
| title | Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. |
| title_full | Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. |
| title_fullStr | Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. |
| title_full_unstemmed | Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. |
| title_short | Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. |
| title_sort | aberration free three dimensional multiphoton imaging of neuronal activity at khz rates |
| work_keys_str_mv | AT botcherbye aberrationfreethreedimensionalmultiphotonimagingofneuronalactivityatkhzrates AT smithc aberrationfreethreedimensionalmultiphotonimagingofneuronalactivityatkhzrates AT kohlm aberrationfreethreedimensionalmultiphotonimagingofneuronalactivityatkhzrates AT debarred aberrationfreethreedimensionalmultiphotonimagingofneuronalactivityatkhzrates AT boothm aberrationfreethreedimensionalmultiphotonimagingofneuronalactivityatkhzrates AT juskaitisr aberrationfreethreedimensionalmultiphotonimagingofneuronalactivityatkhzrates AT paulseno aberrationfreethreedimensionalmultiphotonimagingofneuronalactivityatkhzrates AT wilsont aberrationfreethreedimensionalmultiphotonimagingofneuronalactivityatkhzrates |