Impaired splicing of phytochrome B pre-mRNA in a novel phyB mutant of Arabidopsis.
Phytochrome is the red/far-red absorbing photoreceptor active in photomorphogenesis, the apoprotein of which is encoded by a small gene family (PHYA, PHYB, PHYC, PHYD and PHYE). A novel phytochrome B-deficient mutant, phyB-103, was isolated from a screen of EMS-mutagenised Arabidopsis M2 seed. phyB-...
| Հիմնական հեղինակներ: | , , |
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| Ձևաչափ: | Journal article |
| Լեզու: | English |
| Հրապարակվել է: |
1995
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| _version_ | 1826298226191368192 |
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| author | Bradley, J Whitelam, G Harberd, N |
| author_facet | Bradley, J Whitelam, G Harberd, N |
| author_sort | Bradley, J |
| collection | OXFORD |
| description | Phytochrome is the red/far-red absorbing photoreceptor active in photomorphogenesis, the apoprotein of which is encoded by a small gene family (PHYA, PHYB, PHYC, PHYD and PHYE). A novel phytochrome B-deficient mutant, phyB-103, was isolated from a screen of EMS-mutagenised Arabidopsis M2 seed. phyB-103 carries a G-to-A base substitution at the 5' splice site +1 G nucleotide of intron 1 of PHYB. The phyB-103 PHYB transcript is larger than the wild-type PHYB transcript and DNA sequence analysis showed that the entire intron is retained in the mature PHYB transcript of phyB-103. Thus the phyB-103 G-to-A substitution prevents intron splicing. The retained intron contains within it an in-frame stop codon, and the predicted PHYB-003 apoprotein thus terminates prematurely. phyB-103 is therefore likely to be a null allele of PHYB, consistent with the observation that the phenotype conferred by phyB-103 is as severe as that conferred by previously described phyB null alleles. |
| first_indexed | 2024-03-07T04:43:38Z |
| format | Journal article |
| id | oxford-uuid:d280afb6-3b02-4491-9dd3-d6b2e4be08ab |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:43:38Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:d280afb6-3b02-4491-9dd3-d6b2e4be08ab2022-03-27T08:04:23ZImpaired splicing of phytochrome B pre-mRNA in a novel phyB mutant of Arabidopsis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d280afb6-3b02-4491-9dd3-d6b2e4be08abEnglishSymplectic Elements at Oxford1995Bradley, JWhitelam, GHarberd, NPhytochrome is the red/far-red absorbing photoreceptor active in photomorphogenesis, the apoprotein of which is encoded by a small gene family (PHYA, PHYB, PHYC, PHYD and PHYE). A novel phytochrome B-deficient mutant, phyB-103, was isolated from a screen of EMS-mutagenised Arabidopsis M2 seed. phyB-103 carries a G-to-A base substitution at the 5' splice site +1 G nucleotide of intron 1 of PHYB. The phyB-103 PHYB transcript is larger than the wild-type PHYB transcript and DNA sequence analysis showed that the entire intron is retained in the mature PHYB transcript of phyB-103. Thus the phyB-103 G-to-A substitution prevents intron splicing. The retained intron contains within it an in-frame stop codon, and the predicted PHYB-003 apoprotein thus terminates prematurely. phyB-103 is therefore likely to be a null allele of PHYB, consistent with the observation that the phenotype conferred by phyB-103 is as severe as that conferred by previously described phyB null alleles. |
| spellingShingle | Bradley, J Whitelam, G Harberd, N Impaired splicing of phytochrome B pre-mRNA in a novel phyB mutant of Arabidopsis. |
| title | Impaired splicing of phytochrome B pre-mRNA in a novel phyB mutant of Arabidopsis. |
| title_full | Impaired splicing of phytochrome B pre-mRNA in a novel phyB mutant of Arabidopsis. |
| title_fullStr | Impaired splicing of phytochrome B pre-mRNA in a novel phyB mutant of Arabidopsis. |
| title_full_unstemmed | Impaired splicing of phytochrome B pre-mRNA in a novel phyB mutant of Arabidopsis. |
| title_short | Impaired splicing of phytochrome B pre-mRNA in a novel phyB mutant of Arabidopsis. |
| title_sort | impaired splicing of phytochrome b pre mrna in a novel phyb mutant of arabidopsis |
| work_keys_str_mv | AT bradleyj impairedsplicingofphytochromebpremrnainanovelphybmutantofarabidopsis AT whitelamg impairedsplicingofphytochromebpremrnainanovelphybmutantofarabidopsis AT harberdn impairedsplicingofphytochromebpremrnainanovelphybmutantofarabidopsis |