Alternating-laser excitation of single molecules.
Single-molecule fluorescence spectroscopy addresses biological mechanisms and enables ultrasensitive diagnostics. We describe a new family of single-molecule fluorescence methods that uses alternating-laser excitation (ALEX) of diffusing or immobilized biomolecules to study their structure, interact...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2005
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| _version_ | 1797096576094568448 |
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| author | Kapanidis, A Laurence, T Lee, N Margeat, E Kong, X Weiss, S |
| author_facet | Kapanidis, A Laurence, T Lee, N Margeat, E Kong, X Weiss, S |
| author_sort | Kapanidis, A |
| collection | OXFORD |
| description | Single-molecule fluorescence spectroscopy addresses biological mechanisms and enables ultrasensitive diagnostics. We describe a new family of single-molecule fluorescence methods that uses alternating-laser excitation (ALEX) of diffusing or immobilized biomolecules to study their structure, interactions, and dynamics. This is accomplished using ratios that report on the distance between and the stoichiometry of fluorophores attached to the molecules of interest. The principle of alternation is compatible with several time scales, allowing monitoring of fast dynamics or simultaneous monitoring of a large number of individual molecules. |
| first_indexed | 2024-03-07T04:43:48Z |
| format | Journal article |
| id | oxford-uuid:d290a76f-6b84-44a3-89d0-9567789f7d83 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:43:48Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:d290a76f-6b84-44a3-89d0-9567789f7d832022-03-27T08:04:54ZAlternating-laser excitation of single molecules.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d290a76f-6b84-44a3-89d0-9567789f7d83EnglishSymplectic Elements at Oxford2005Kapanidis, ALaurence, TLee, NMargeat, EKong, XWeiss, SSingle-molecule fluorescence spectroscopy addresses biological mechanisms and enables ultrasensitive diagnostics. We describe a new family of single-molecule fluorescence methods that uses alternating-laser excitation (ALEX) of diffusing or immobilized biomolecules to study their structure, interactions, and dynamics. This is accomplished using ratios that report on the distance between and the stoichiometry of fluorophores attached to the molecules of interest. The principle of alternation is compatible with several time scales, allowing monitoring of fast dynamics or simultaneous monitoring of a large number of individual molecules. |
| spellingShingle | Kapanidis, A Laurence, T Lee, N Margeat, E Kong, X Weiss, S Alternating-laser excitation of single molecules. |
| title | Alternating-laser excitation of single molecules. |
| title_full | Alternating-laser excitation of single molecules. |
| title_fullStr | Alternating-laser excitation of single molecules. |
| title_full_unstemmed | Alternating-laser excitation of single molecules. |
| title_short | Alternating-laser excitation of single molecules. |
| title_sort | alternating laser excitation of single molecules |
| work_keys_str_mv | AT kapanidisa alternatinglaserexcitationofsinglemolecules AT laurencet alternatinglaserexcitationofsinglemolecules AT leen alternatinglaserexcitationofsinglemolecules AT margeate alternatinglaserexcitationofsinglemolecules AT kongx alternatinglaserexcitationofsinglemolecules AT weisss alternatinglaserexcitationofsinglemolecules |