Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype
Atypical memory B cells (aMBCs) are found in elevated numbers in individuals exposed to malaria. A key question is whether malaria induces aMBCs as a result of exposure to Ag, or non-Ag-specific mechanisms. We identified Plasmodium and bystander tetanus toxoid (TT) specific B cells in individuals fr...
| Главные авторы: | , , , , , , , , , , |
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| Формат: | Journal article |
| Язык: | English |
| Опубликовано: |
Wiley
2020
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| _version_ | 1826298303602491392 |
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| author | Aye, R Sutton, HJ Nduati, EW Kai, O Mwacharo, J Musyoki, J Otieno, E Wambua, J Bejon, P Cockburn, IA Ndungu, FM |
| author_facet | Aye, R Sutton, HJ Nduati, EW Kai, O Mwacharo, J Musyoki, J Otieno, E Wambua, J Bejon, P Cockburn, IA Ndungu, FM |
| author_sort | Aye, R |
| collection | OXFORD |
| description | Atypical memory B cells (aMBCs) are found in elevated numbers in individuals exposed to malaria. A key question is whether malaria induces aMBCs as a result of exposure to Ag, or non-Ag-specific mechanisms. We identified Plasmodium and bystander tetanus toxoid (TT) specific B cells in individuals from areas of previous and persistent exposure to malaria using tetramers. Malaria-specific B cells were more likely to be aMBCs than TT-specific B cells. However, TT-specific B cells from individuals with continuous exposure to malaria were more likely to be aMBCs than TT-specific B cells in individuals from areas where transmission has ceased. Finally, sequences of BCRs specific for a blood stage malaria-Ag were more highly mutated than sequences from TT-specific BCRs and under strong negative selection, indicative of ongoing antigenic pressure. Our data suggest both persistent Ag exposure and the inflammatory environment shape the B-cell response to malaria and bystander Ags. |
| first_indexed | 2024-03-07T04:44:47Z |
| format | Journal article |
| id | oxford-uuid:d2db813c-778a-4e90-b3cf-0ca7d5fa7ff4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:44:47Z |
| publishDate | 2020 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:d2db813c-778a-4e90-b3cf-0ca7d5fa7ff42022-03-27T08:07:13ZMalaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotypeJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d2db813c-778a-4e90-b3cf-0ca7d5fa7ff4EnglishSymplectic ElementsWiley2020Aye, RSutton, HJNduati, EWKai, OMwacharo, JMusyoki, JOtieno, EWambua, JBejon, PCockburn, IANdungu, FMAtypical memory B cells (aMBCs) are found in elevated numbers in individuals exposed to malaria. A key question is whether malaria induces aMBCs as a result of exposure to Ag, or non-Ag-specific mechanisms. We identified Plasmodium and bystander tetanus toxoid (TT) specific B cells in individuals from areas of previous and persistent exposure to malaria using tetramers. Malaria-specific B cells were more likely to be aMBCs than TT-specific B cells. However, TT-specific B cells from individuals with continuous exposure to malaria were more likely to be aMBCs than TT-specific B cells in individuals from areas where transmission has ceased. Finally, sequences of BCRs specific for a blood stage malaria-Ag were more highly mutated than sequences from TT-specific BCRs and under strong negative selection, indicative of ongoing antigenic pressure. Our data suggest both persistent Ag exposure and the inflammatory environment shape the B-cell response to malaria and bystander Ags. |
| spellingShingle | Aye, R Sutton, HJ Nduati, EW Kai, O Mwacharo, J Musyoki, J Otieno, E Wambua, J Bejon, P Cockburn, IA Ndungu, FM Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype |
| title | Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype |
| title_full | Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype |
| title_fullStr | Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype |
| title_full_unstemmed | Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype |
| title_short | Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype |
| title_sort | malaria exposure drives both cognate and bystander human b cells to adopt an atypical phenotype |
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