Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains
The course of hypertension remains poorly understood, although impairment of the sympathetic nervous systems is thought to play a role in its aetiology. In this study, RNA-sequencing (RNAseq) was used to identify transcriptomal differences in the sympathetic stellate ganglia between 16-week-old norm...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Nature Research
2018
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| _version_ | 1797096673816608768 |
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| author | Davis, H Bardsley, E Paterson, D |
| author_facet | Davis, H Bardsley, E Paterson, D |
| author_sort | Davis, H |
| collection | OXFORD |
| description | The course of hypertension remains poorly understood, although impairment of the sympathetic nervous systems is thought to play a role in its aetiology. In this study, RNA-sequencing (RNAseq) was used to identify transcriptomal differences in the sympathetic stellate ganglia between 16-week-old normotensive Wistar rats and spontaneously hypertensive rats (SHR). Sequencing quality was assessed by FastQC and quasi-mapping rate by Salmon. Differential expression results were confirmed by real time reverse transcriptase Quantitative Polymerase Chain Reaction (qRT-PCR). RNAseq analysis was found to be predictive and representative of transcriptomal changes when compared to qRT-PCR by correlation analysis. Whether these changes underpin physiological sympathetic phenotypes associated with hypertension remains to be established, however this dataset identifies lead transcripts as a priori targets for further investigation. |
| first_indexed | 2024-03-07T04:44:53Z |
| format | Journal article |
| id | oxford-uuid:d2e61f2b-fc94-436e-96e4-92c60ffaef86 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:44:53Z |
| publishDate | 2018 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:d2e61f2b-fc94-436e-96e4-92c60ffaef862022-03-27T08:07:25ZTranscriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strainsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d2e61f2b-fc94-436e-96e4-92c60ffaef86EnglishSymplectic Elements at OxfordNature Research2018Davis, HBardsley, EPaterson, DThe course of hypertension remains poorly understood, although impairment of the sympathetic nervous systems is thought to play a role in its aetiology. In this study, RNA-sequencing (RNAseq) was used to identify transcriptomal differences in the sympathetic stellate ganglia between 16-week-old normotensive Wistar rats and spontaneously hypertensive rats (SHR). Sequencing quality was assessed by FastQC and quasi-mapping rate by Salmon. Differential expression results were confirmed by real time reverse transcriptase Quantitative Polymerase Chain Reaction (qRT-PCR). RNAseq analysis was found to be predictive and representative of transcriptomal changes when compared to qRT-PCR by correlation analysis. Whether these changes underpin physiological sympathetic phenotypes associated with hypertension remains to be established, however this dataset identifies lead transcripts as a priori targets for further investigation. |
| spellingShingle | Davis, H Bardsley, E Paterson, D Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains |
| title | Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains |
| title_full | Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains |
| title_fullStr | Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains |
| title_full_unstemmed | Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains |
| title_short | Transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains |
| title_sort | transcriptional profiling of stellate ganglia from normotensive and spontaneously hypertensive rat strains |
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