The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum.
Plasmodium species ex vivo sensitivity assay protocols differ in the requirement for leukocyte removal before culturing. This study shows that the presence of leukocytes significantly increases the 50% inhibitory concentration (IC₅₀) of P. vivax and P. falciparum to artesunate and chloroquine relati...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2011
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| _version_ | 1797096682169565184 |
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| author | Kaewpongsri, S Sriprawat, K Suwanarusk, R Kyle, D Lek-Uthai, U Leimanis, M Lwin, K Phyo, A Zwang, J Russell, B Nosten, F Renia, L |
| author_facet | Kaewpongsri, S Sriprawat, K Suwanarusk, R Kyle, D Lek-Uthai, U Leimanis, M Lwin, K Phyo, A Zwang, J Russell, B Nosten, F Renia, L |
| author_sort | Kaewpongsri, S |
| collection | OXFORD |
| description | Plasmodium species ex vivo sensitivity assay protocols differ in the requirement for leukocyte removal before culturing. This study shows that the presence of leukocytes significantly increases the 50% inhibitory concentration (IC₅₀) of P. vivax and P. falciparum to artesunate and chloroquine relative to results with the paired leukocyte-free treatment. Although leukocyte removal is not an essential requirement for the conduct of ex vivo assays, its use has important implications for the interpretation of temporal and spatial antimalarial sensitivity data. |
| first_indexed | 2024-03-07T04:45:00Z |
| format | Journal article |
| id | oxford-uuid:d2f2f009-e781-4f47-9505-1e8788d85b67 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:45:00Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:d2f2f009-e781-4f47-9505-1e8788d85b672022-03-27T08:07:49ZThe presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d2f2f009-e781-4f47-9505-1e8788d85b67EnglishSymplectic Elements at Oxford2011Kaewpongsri, SSriprawat, KSuwanarusk, RKyle, DLek-Uthai, ULeimanis, MLwin, KPhyo, AZwang, JRussell, BNosten, FRenia, LPlasmodium species ex vivo sensitivity assay protocols differ in the requirement for leukocyte removal before culturing. This study shows that the presence of leukocytes significantly increases the 50% inhibitory concentration (IC₅₀) of P. vivax and P. falciparum to artesunate and chloroquine relative to results with the paired leukocyte-free treatment. Although leukocyte removal is not an essential requirement for the conduct of ex vivo assays, its use has important implications for the interpretation of temporal and spatial antimalarial sensitivity data. |
| spellingShingle | Kaewpongsri, S Sriprawat, K Suwanarusk, R Kyle, D Lek-Uthai, U Leimanis, M Lwin, K Phyo, A Zwang, J Russell, B Nosten, F Renia, L The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum. |
| title | The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum. |
| title_full | The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum. |
| title_fullStr | The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum. |
| title_full_unstemmed | The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum. |
| title_short | The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum. |
| title_sort | presence of leukocytes in ex vivo assays significantly increases the 50 percent inhibitory concentrations of artesunate and chloroquine against plasmodium vivax and plasmodium falciparum |
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