Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS
We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAP...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal article |
| Publicado: |
BMC
2020
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| _version_ | 1826298420192608256 |
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| author | Liu, Y Cheng, J Siejka-Zielinska, P Weldon, C Roberts, H Lopopolo, M Magri, A D'Arienzo, V Harris, JM McKeating, J Song, C |
| author_facet | Liu, Y Cheng, J Siejka-Zielinska, P Weldon, C Roberts, H Lopopolo, M Magri, A D'Arienzo, V Harris, JM McKeating, J Song, C |
| author_sort | Liu, Y |
| collection | OXFORD |
| description | We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome. |
| first_indexed | 2024-03-07T04:46:33Z |
| format | Journal article |
| id | oxford-uuid:d380f45c-af7c-45a8-9b59-77880951059f |
| institution | University of Oxford |
| last_indexed | 2024-03-07T04:46:33Z |
| publishDate | 2020 |
| publisher | BMC |
| record_format | dspace |
| spelling | oxford-uuid:d380f45c-af7c-45a8-9b59-77880951059f2022-03-27T08:11:37ZAccurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPSJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d380f45c-af7c-45a8-9b59-77880951059fSymplectic ElementsBMC2020Liu, YCheng, JSiejka-Zielinska, PWeldon, CRoberts, HLopopolo, MMagri, AD'Arienzo, VHarris, JMMcKeating, JSong, CWe present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome. |
| spellingShingle | Liu, Y Cheng, J Siejka-Zielinska, P Weldon, C Roberts, H Lopopolo, M Magri, A D'Arienzo, V Harris, JM McKeating, J Song, C Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS |
| title | Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS |
| title_full | Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS |
| title_fullStr | Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS |
| title_full_unstemmed | Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS |
| title_short | Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS |
| title_sort | accurate targeted long read dna methylation and hydroxymethylation sequencing with taps |
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