Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis
Sepsis is a complex syndrome related to an infection-induced exaggerated inflammatory response, which is associated with a high mortality. Granzymes (Gzm) are proteases mainly found in cytotoxic lymphocytes that not only have a role in target cell death, but also as mediators of infection and inflam...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2021
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| _version_ | 1797096796152922112 |
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| author | García-Laorden, MI Hoogendijk, AJ Wiewel, MA van Vught, LA Schultz, MJ Bovenschen, N de Vos, AF van der Poll, T |
| author_facet | García-Laorden, MI Hoogendijk, AJ Wiewel, MA van Vught, LA Schultz, MJ Bovenschen, N de Vos, AF van der Poll, T |
| author_sort | García-Laorden, MI |
| collection | OXFORD |
| description | Sepsis is a complex syndrome related to an infection-induced exaggerated inflammatory response, which is associated with a high mortality. Granzymes (Gzm) are proteases mainly found in cytotoxic lymphocytes that not only have a role in target cell death, but also as mediators of infection and inflammation. In this study we sought to analyse the intracellular expression of GzmA, B, M and K by flow cytometry in diverse blood lymphocyte populations from 22 sepsis patients, 12 non-infected intensive care unit (ICU) patients and 32 healthy controls. Additionally, we measured GzmA and B plasma levels. Both groups of patients presented decreased percentage of natural killer (NK) cells expressing GzmA, B and M relative to healthy controls, while sepsis patients showed an increased proportion of CD8+ T cells expressing GzmB compared to controls. Expression of GzmK remained relatively unaltered between groups. Extracellular levels of GzmB were increased in non-infected ICU patients relative to sepsis patients and healthy controls. Our results show differential alterations in intracellular expression of Gzm in sepsis patients and non-infected critically ill patients compared to healthy individuals depending on the lymphocyte population and on the Gzm.
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| first_indexed | 2024-03-07T04:46:40Z |
| format | Journal article |
| id | oxford-uuid:d38820b1-2b25-49c2-9c2b-72395a080eb7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:46:40Z |
| publishDate | 2021 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:d38820b1-2b25-49c2-9c2b-72395a080eb72022-03-27T08:11:57ZIntracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d38820b1-2b25-49c2-9c2b-72395a080eb7EnglishSymplectic ElementsOxford University Press2021García-Laorden, MIHoogendijk, AJWiewel, MAvan Vught, LASchultz, MJBovenschen, Nde Vos, AFvan der Poll, TSepsis is a complex syndrome related to an infection-induced exaggerated inflammatory response, which is associated with a high mortality. Granzymes (Gzm) are proteases mainly found in cytotoxic lymphocytes that not only have a role in target cell death, but also as mediators of infection and inflammation. In this study we sought to analyse the intracellular expression of GzmA, B, M and K by flow cytometry in diverse blood lymphocyte populations from 22 sepsis patients, 12 non-infected intensive care unit (ICU) patients and 32 healthy controls. Additionally, we measured GzmA and B plasma levels. Both groups of patients presented decreased percentage of natural killer (NK) cells expressing GzmA, B and M relative to healthy controls, while sepsis patients showed an increased proportion of CD8+ T cells expressing GzmB compared to controls. Expression of GzmK remained relatively unaltered between groups. Extracellular levels of GzmB were increased in non-infected ICU patients relative to sepsis patients and healthy controls. Our results show differential alterations in intracellular expression of Gzm in sepsis patients and non-infected critically ill patients compared to healthy individuals depending on the lymphocyte population and on the Gzm. |
| spellingShingle | García-Laorden, MI Hoogendijk, AJ Wiewel, MA van Vught, LA Schultz, MJ Bovenschen, N de Vos, AF van der Poll, T Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis |
| title | Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis |
| title_full | Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis |
| title_fullStr | Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis |
| title_full_unstemmed | Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis |
| title_short | Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis |
| title_sort | intracellular expression of granzymes a b k and m in blood lymphocyte subsets of critically ill patients with or without sepsis |
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