Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage)
The total thiols in a medium containing authentic biological sample (articular cartilage) has been determined electrochemically. The detection strategy utilises the electrochemical adaptation of the Ellman's reaction process via the electrochemically initiated reaction of thiols with diethyl-p-...
| Main Authors: | , , , , |
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| 格式: | Journal article |
| 语言: | English |
| 出版: |
2003
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| _version_ | 1826298459376844800 |
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| author | Nekrassova, O Lawrence, N Lee, R Urban, J Compton, R |
| author_facet | Nekrassova, O Lawrence, N Lee, R Urban, J Compton, R |
| author_sort | Nekrassova, O |
| collection | OXFORD |
| description | The total thiols in a medium containing authentic biological sample (articular cartilage) has been determined electrochemically. The detection strategy utilises the electrochemical adaptation of the Ellman's reaction process via the electrochemically initiated reaction of thiols with diethyl-p-phenylenediamine. The strategy has been examined over a biologically significant pH range and the behaviour observed rationalised. The detection of thiols within the biological sample is shown to proceed with micromolar levels of thiol determined within the sample. |
| first_indexed | 2024-03-07T04:47:10Z |
| format | Journal article |
| id | oxford-uuid:d3adc38c-c1ac-4d32-a454-f3361f9e1fa7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:47:10Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:d3adc38c-c1ac-4d32-a454-f3361f9e1fa72022-03-27T08:13:05ZElectroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage)Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d3adc38c-c1ac-4d32-a454-f3361f9e1fa7EnglishSymplectic Elements at Oxford2003Nekrassova, OLawrence, NLee, RUrban, JCompton, RThe total thiols in a medium containing authentic biological sample (articular cartilage) has been determined electrochemically. The detection strategy utilises the electrochemical adaptation of the Ellman's reaction process via the electrochemically initiated reaction of thiols with diethyl-p-phenylenediamine. The strategy has been examined over a biologically significant pH range and the behaviour observed rationalised. The detection of thiols within the biological sample is shown to proceed with micromolar levels of thiol determined within the sample. |
| spellingShingle | Nekrassova, O Lawrence, N Lee, R Urban, J Compton, R Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage) |
| title | Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage) |
| title_full | Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage) |
| title_fullStr | Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage) |
| title_full_unstemmed | Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage) |
| title_short | Electroanalytical adaptation of the classical Ellman's Assay: Determination of total thiols in incubation media from a biological sample (excised articular cartilage) |
| title_sort | electroanalytical adaptation of the classical ellman s assay determination of total thiols in incubation media from a biological sample excised articular cartilage |
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