Effective induction of HIV-specific CTL by multi-epitope using gene gun in a combined vaccination regime.
Reliable and effective induction of cytotoxic T-lymphocytes (CTL) is one of the prime objectives of vaccine research. Previously, novel HIV vaccine candidates were constructed as a string of CTL epitopes (20 human, 3 macaque and 1 mouse) delivered using a DNA vector [Hanke T, Schneider J, Gilbert SG...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
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1999
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| _version_ | 1797096859368423424 |
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| author | Hanke, T Neumann, VC Blanchard, T Sweeney, P Hill, A Smith, G McMichael, A |
| author_facet | Hanke, T Neumann, VC Blanchard, T Sweeney, P Hill, A Smith, G McMichael, A |
| author_sort | Hanke, T |
| collection | OXFORD |
| description | Reliable and effective induction of cytotoxic T-lymphocytes (CTL) is one of the prime objectives of vaccine research. Previously, novel HIV vaccine candidates were constructed as a string of CTL epitopes (20 human, 3 macaque and 1 mouse) delivered using a DNA vector [Hanke T, Schneider J, Gilbert SG, Hill AVS, McMichael A. DNA multi-CTL epitope vaccines for HIV and Plasmodium falciparum: immunogenicity in mice. Vaccine 1998;16:426-435.] or modified vaccinia Ankara (MVA [Hanke T, Blanchard TJ, Schneider J, Ogg GS, Tan R, Becker MSC, Gilbert SG, Hill AVS, Smith GL, McMichael A. Immunogenicities of intravenous and intramuscular administrations of MVA-based multi-CTL epitope vaccine for HIV in mice. J Gen Virol 1998;79:83-90.]), i.e. vaccine vehicles acceptable for use in humans. In mice, a single intramuscular (i.m.) needle injection of either vaccine alone elicited good CTL responses. Here, it is demonstrated that the multi-epitope DNA also induced CTL when delivered intradermally using the Accell gene gun. The CTL responses increased after re-immunization and after three deliveries were comparable to those induced by a single i.m. injection. Recent evidence indicates that combining routes and vaccine vehicles enhances the immunogenicity of vaccine-delivered or -encoded antigens. Here, it is shown that administration of DNA by an i.m. priming/gene gun boosting more efficiently induced CTL than gene gun priming/i.m. boosting. A similar increment was obtained by sequential vaccinations using a gene gun-delivered DNA followed by recombinant MVA. Thus particular sequences of routes or vaccine vehicles rather than simple prime-boost delivery of a single vaccine is critical for an effective elicitation of CTL. |
| first_indexed | 2024-03-07T04:47:32Z |
| format | Journal article |
| id | oxford-uuid:d3d3a614-af99-4905-b03f-44a8a3902210 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:47:32Z |
| publishDate | 1999 |
| record_format | dspace |
| spelling | oxford-uuid:d3d3a614-af99-4905-b03f-44a8a39022102022-03-27T08:14:01ZEffective induction of HIV-specific CTL by multi-epitope using gene gun in a combined vaccination regime.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d3d3a614-af99-4905-b03f-44a8a3902210EnglishSymplectic Elements at Oxford1999Hanke, TNeumann, VCBlanchard, TSweeney, PHill, ASmith, GMcMichael, AReliable and effective induction of cytotoxic T-lymphocytes (CTL) is one of the prime objectives of vaccine research. Previously, novel HIV vaccine candidates were constructed as a string of CTL epitopes (20 human, 3 macaque and 1 mouse) delivered using a DNA vector [Hanke T, Schneider J, Gilbert SG, Hill AVS, McMichael A. DNA multi-CTL epitope vaccines for HIV and Plasmodium falciparum: immunogenicity in mice. Vaccine 1998;16:426-435.] or modified vaccinia Ankara (MVA [Hanke T, Blanchard TJ, Schneider J, Ogg GS, Tan R, Becker MSC, Gilbert SG, Hill AVS, Smith GL, McMichael A. Immunogenicities of intravenous and intramuscular administrations of MVA-based multi-CTL epitope vaccine for HIV in mice. J Gen Virol 1998;79:83-90.]), i.e. vaccine vehicles acceptable for use in humans. In mice, a single intramuscular (i.m.) needle injection of either vaccine alone elicited good CTL responses. Here, it is demonstrated that the multi-epitope DNA also induced CTL when delivered intradermally using the Accell gene gun. The CTL responses increased after re-immunization and after three deliveries were comparable to those induced by a single i.m. injection. Recent evidence indicates that combining routes and vaccine vehicles enhances the immunogenicity of vaccine-delivered or -encoded antigens. Here, it is shown that administration of DNA by an i.m. priming/gene gun boosting more efficiently induced CTL than gene gun priming/i.m. boosting. A similar increment was obtained by sequential vaccinations using a gene gun-delivered DNA followed by recombinant MVA. Thus particular sequences of routes or vaccine vehicles rather than simple prime-boost delivery of a single vaccine is critical for an effective elicitation of CTL. |
| spellingShingle | Hanke, T Neumann, VC Blanchard, T Sweeney, P Hill, A Smith, G McMichael, A Effective induction of HIV-specific CTL by multi-epitope using gene gun in a combined vaccination regime. |
| title | Effective induction of HIV-specific CTL by multi-epitope using gene gun in a combined vaccination regime. |
| title_full | Effective induction of HIV-specific CTL by multi-epitope using gene gun in a combined vaccination regime. |
| title_fullStr | Effective induction of HIV-specific CTL by multi-epitope using gene gun in a combined vaccination regime. |
| title_full_unstemmed | Effective induction of HIV-specific CTL by multi-epitope using gene gun in a combined vaccination regime. |
| title_short | Effective induction of HIV-specific CTL by multi-epitope using gene gun in a combined vaccination regime. |
| title_sort | effective induction of hiv specific ctl by multi epitope using gene gun in a combined vaccination regime |
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