Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4.
CHD4, the core subunit of the Nucleosome Remodelling and Deacetylase (NuRD) complex, is a chromatin remodelling ATPase that, in addition to a helicase domain, harbors tandem plant homeo finger and chromo domains. By using a panel of domain constructs we dissect their roles and demonstrate that DNA b...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1797096873496936448 |
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| author | Morra, R Lee, B Shaw, H Tuma, R Mancini, E |
| author_facet | Morra, R Lee, B Shaw, H Tuma, R Mancini, E |
| author_sort | Morra, R |
| collection | OXFORD |
| description | CHD4, the core subunit of the Nucleosome Remodelling and Deacetylase (NuRD) complex, is a chromatin remodelling ATPase that, in addition to a helicase domain, harbors tandem plant homeo finger and chromo domains. By using a panel of domain constructs we dissect their roles and demonstrate that DNA binding, histone binding and ATPase activities are allosterically regulated. Molecular shape reconstruction from small-angle X-ray scattering reveals extensive domain-domain interactions, which provide a structural explanation for the regulation of CHD4 activities by intramolecular domain communication. Our results demonstrate functional interdependency between domains within a chromatin remodeller. |
| first_indexed | 2024-03-07T04:47:45Z |
| format | Journal article |
| id | oxford-uuid:d3e51e43-31bf-4950-9473-688875a96065 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:47:45Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:d3e51e43-31bf-4950-9473-688875a960652022-03-27T08:14:30ZConcerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d3e51e43-31bf-4950-9473-688875a96065EnglishSymplectic Elements at Oxford2012Morra, RLee, BShaw, HTuma, RMancini, ECHD4, the core subunit of the Nucleosome Remodelling and Deacetylase (NuRD) complex, is a chromatin remodelling ATPase that, in addition to a helicase domain, harbors tandem plant homeo finger and chromo domains. By using a panel of domain constructs we dissect their roles and demonstrate that DNA binding, histone binding and ATPase activities are allosterically regulated. Molecular shape reconstruction from small-angle X-ray scattering reveals extensive domain-domain interactions, which provide a structural explanation for the regulation of CHD4 activities by intramolecular domain communication. Our results demonstrate functional interdependency between domains within a chromatin remodeller. |
| spellingShingle | Morra, R Lee, B Shaw, H Tuma, R Mancini, E Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4. |
| title | Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4. |
| title_full | Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4. |
| title_fullStr | Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4. |
| title_full_unstemmed | Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4. |
| title_short | Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4. |
| title_sort | concerted action of the phd chromo and motor domains regulates the human chromatin remodelling atpase chd4 |
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