A simple backscattering microscope for fast tracking of biological molecules.
Recent developments in techniques for observing single molecules under light microscopes have helped reveal the mechanisms by which molecular machines work. A wide range of markers can be used to detect molecules, from single fluorophores to micron sized markers, depending on the research interest....
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2010
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| _version_ | 1797096934876381184 |
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| author | Sowa, Y Steel, B Berry, R |
| author_facet | Sowa, Y Steel, B Berry, R |
| author_sort | Sowa, Y |
| collection | OXFORD |
| description | Recent developments in techniques for observing single molecules under light microscopes have helped reveal the mechanisms by which molecular machines work. A wide range of markers can be used to detect molecules, from single fluorophores to micron sized markers, depending on the research interest. Here, we present a new and simple objective-type backscattering microscope to track gold nanoparticles with nanometer and microsecond resolution. The total noise of our system in a 55 kHz bandwidth is ~0.6 nm per axis, sufficient to measure molecular movement. We found our backscattering microscopy to be useful not only for in vitro but also for in vivo experiments because of lower background scattering from cells than in conventional dark-field microscopy. We demonstrate the application of this technique to measuring the motion of a biological rotary molecular motor, the bacterial flagellar motor, in live Escherichia coli cells. |
| first_indexed | 2024-03-07T04:48:33Z |
| format | Journal article |
| id | oxford-uuid:d42a8d0d-9b9e-410a-a34d-06deb5421a70 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:48:33Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:d42a8d0d-9b9e-410a-a34d-06deb5421a702022-03-27T08:16:22ZA simple backscattering microscope for fast tracking of biological molecules.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d42a8d0d-9b9e-410a-a34d-06deb5421a70EnglishSymplectic Elements at Oxford2010Sowa, YSteel, BBerry, RRecent developments in techniques for observing single molecules under light microscopes have helped reveal the mechanisms by which molecular machines work. A wide range of markers can be used to detect molecules, from single fluorophores to micron sized markers, depending on the research interest. Here, we present a new and simple objective-type backscattering microscope to track gold nanoparticles with nanometer and microsecond resolution. The total noise of our system in a 55 kHz bandwidth is ~0.6 nm per axis, sufficient to measure molecular movement. We found our backscattering microscopy to be useful not only for in vitro but also for in vivo experiments because of lower background scattering from cells than in conventional dark-field microscopy. We demonstrate the application of this technique to measuring the motion of a biological rotary molecular motor, the bacterial flagellar motor, in live Escherichia coli cells. |
| spellingShingle | Sowa, Y Steel, B Berry, R A simple backscattering microscope for fast tracking of biological molecules. |
| title | A simple backscattering microscope for fast tracking of biological molecules. |
| title_full | A simple backscattering microscope for fast tracking of biological molecules. |
| title_fullStr | A simple backscattering microscope for fast tracking of biological molecules. |
| title_full_unstemmed | A simple backscattering microscope for fast tracking of biological molecules. |
| title_short | A simple backscattering microscope for fast tracking of biological molecules. |
| title_sort | simple backscattering microscope for fast tracking of biological molecules |
| work_keys_str_mv | AT soway asimplebackscatteringmicroscopeforfasttrackingofbiologicalmolecules AT steelb asimplebackscatteringmicroscopeforfasttrackingofbiologicalmolecules AT berryr asimplebackscatteringmicroscopeforfasttrackingofbiologicalmolecules AT soway simplebackscatteringmicroscopeforfasttrackingofbiologicalmolecules AT steelb simplebackscatteringmicroscopeforfasttrackingofbiologicalmolecules AT berryr simplebackscatteringmicroscopeforfasttrackingofbiologicalmolecules |