The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA
AbstractTermination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Udgivet: |
Nature Publishing Group
2017
|
| _version_ | 1826311461423546368 |
|---|---|
| author | Wittmann, S Renner, M Watts, B Adams, O Huseyin, M Baejen, C El Omari, K Kilchert, C Heo, D Kecman, T Cramer, P Grimes, J Vasiljeva, L Vasilieva, L |
| author_facet | Wittmann, S Renner, M Watts, B Adams, O Huseyin, M Baejen, C El Omari, K Kilchert, C Heo, D Kecman, T Cramer, P Grimes, J Vasiljeva, L Vasilieva, L |
| author_sort | Wittmann, S |
| collection | OXFORD |
| description | AbstractTermination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed that a common feature of these proteins is a conserved domain that interacts with the phosphorylated C-terminus of Pol II (CTD-interacting domain, CID). However, the mechanism by which transcription termination is achieved is not understood. Using genome-wide methods, here we show that the fission yeast CID-protein Seb1 is essential for termination of protein-coding and non-coding genes through interaction with S2-phosphorylated Pol II and nascent RNA. Furthermore, we present the crystal structures of the Seb1 CTD- and RNA-binding modules. Unexpectedly, the latter reveals an intertwined two-domain arrangement of a canonical RRM and second domain. These results provide important insights into the mechanism underlying eukaryotic transcription termination. |
| first_indexed | 2024-03-07T08:10:11Z |
| format | Journal article |
| id | oxford-uuid:d4433d0f-d17c-4df6-8691-f1ce25ed1a97 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T08:10:11Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:d4433d0f-d17c-4df6-8691-f1ce25ed1a972023-11-21T10:02:10ZThe conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNAJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d4433d0f-d17c-4df6-8691-f1ce25ed1a97Symplectic Elements at OxfordNature Publishing Group2017Wittmann, SRenner, MWatts, BAdams, OHuseyin, MBaejen, CEl Omari, KKilchert, CHeo, DKecman, TCramer, PGrimes, JVasiljeva, LVasilieva, LAbstractTermination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed that a common feature of these proteins is a conserved domain that interacts with the phosphorylated C-terminus of Pol II (CTD-interacting domain, CID). However, the mechanism by which transcription termination is achieved is not understood. Using genome-wide methods, here we show that the fission yeast CID-protein Seb1 is essential for termination of protein-coding and non-coding genes through interaction with S2-phosphorylated Pol II and nascent RNA. Furthermore, we present the crystal structures of the Seb1 CTD- and RNA-binding modules. Unexpectedly, the latter reveals an intertwined two-domain arrangement of a canonical RRM and second domain. These results provide important insights into the mechanism underlying eukaryotic transcription termination. |
| spellingShingle | Wittmann, S Renner, M Watts, B Adams, O Huseyin, M Baejen, C El Omari, K Kilchert, C Heo, D Kecman, T Cramer, P Grimes, J Vasiljeva, L Vasilieva, L The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA |
| title | The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA |
| title_full | The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA |
| title_fullStr | The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA |
| title_full_unstemmed | The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA |
| title_short | The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA |
| title_sort | conserved protein seb1 drives transcription termination by binding rna polymerase ii and nascent rna |
| work_keys_str_mv | AT wittmanns theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT rennerm theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT wattsb theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT adamso theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT huseyinm theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT baejenc theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT elomarik theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT kilchertc theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT heod theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT kecmant theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT cramerp theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT grimesj theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT vasiljeval theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT vasilieval theconservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT wittmanns conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT rennerm conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT wattsb conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT adamso conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT huseyinm conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT baejenc conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT elomarik conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT kilchertc conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT heod conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT kecmant conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT cramerp conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT grimesj conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT vasiljeval conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna AT vasilieval conservedproteinseb1drivestranscriptionterminationbybindingrnapolymeraseiiandnascentrna |